健脾除湿汤对脾虚型慢性湿疹小鼠血清IL-4、IFN-γ的干预作用研究

Effect of Jianpi Dedampness Decoction on Serum IL-4, IFN-γ in Mice with Chronic Eczema of Spleen Deficiency Type

摘要: 目的：探讨健脾除湿汤对脾虚型慢性湿疹小鼠血清IL-4、IFN-γ的干预作用。方法：将48只雄性Wistar小鼠分成六组(空白对照组、模型对照组、低剂量灌胃组、中剂量灌胃组、高剂量灌胃组、氯雷他定组)，以化学致炎剂2,4-二硝基氯苯(DNCB)建立小鼠脾虚型慢性湿疹模型，使用健脾除湿汤进行模型干预，采用灌胃的途径给药，观察小鼠血清中IL-4、IFN-γ水平的变化。结果：脾虚型慢性湿疹小鼠的血清IL-4升高，IFN-γ降低；健脾除湿汤能使脾虚型慢性湿疹小鼠的血清IL-4降低，IFN-γ升高，其效果优于氯雷他定组。结论：健脾除湿汤通过对血清IL-4、IFN-γ的调节治疗脾虚型慢性湿疹，高剂量组效果好。

Abstract: Objective: To investigate the intervention effect of Jianpi Dedampness decoction on serum IL-4, IFN-γ in mice with chronic eczema of spleen deficiency type. Methods: 48 male Wistar mice were divided into six groups (blank control group, model control group, low dose gastric perfusion group, middle dose gastric perfusion group, high dose gastric perfusion group and loratadine group). The chronic eczema model of spleen deficiency type was established with chemical in-flammatory agent 2,4-dinitrochlorobenzene (DNCB). The model intervention was carried out with Jianpi Dedampness decoction, and the changes of IL-4, IFN-γ level in serum of mice were observed by intragastric administration. Results: The results of chronic eczema of spleen deficiency type were found in mice with chronic eczema of spleen deficiency type. Serum IL-4 increased and IFN-γ decreased, and Jianpi Dedampness decoction could decrease serum IL-4 and increase IFN-γ in mice with chronic eczema of spleen deficiency type, which was better than that of loratadine group. Conclusion: Jianpi Dedampness decoction can treat chronic eczema of spleen deficiency type by regulating serum IL-4, IFN-γ.