红景天愈伤组织诱导及红景天苷积累的研究
Study on Callus Induction and Salidroside Accumulation in Rhodiola L.
DOI: 10.12677/BR.2019.86055, PDF,    科研立项经费支持
作者: 张国珍：四川省自然资源科学研究院，四川 成都；成都大学农业农村部杂粮加工重点实验室，四川 成都；谢 洁：四川师范大学生命科学学院，四川 成都；秦小波：四川省自然资源科学研究院，四川 成都；成都大学农业农村部杂粮加工重点实验室，四川 成都；四川大学生命科学学院，生物资源与生态环境教育部重点实验室，四川 成都；牛 蓓, 时小东：成都大学农业农村部杂粮加工重点实验室，四川 成都；胡冬梅：四川大学生命科学学院，生物资源与生态环境教育部重点实验室，四川 成都；樊莉娟：四川省自然资源科学研究院，四川 成都；四川大学生命科学学院，生物资源与生态环境教育部重点实验室，四川 成都；徐心艺：电子科技大学实验中学，四川 成都；秦一菲：成都市磨子桥小学，四川 成都
关键词: 红景天；愈伤组织；红景天苷；植物生长激素；甲基茉莉酸；Rhodiola L.； Callus； Salidroside； Plant Growth Hormone； Methyl Jasmonate
摘要: 通过对红景天叶片、茎、根产生愈伤组织的培养，建立了培养周期短、愈伤组织生长效率高的培养体系。利用多因子正交实验设计及分析，筛选出最佳愈伤组织诱导培养基是MS + 2.0 mg/L 6-BA + 0.2 mg/L NAA + 0.1 mg/L 2,4-D。通过甲基茉莉酸的添加研究诱导愈伤组织生物量及药效物质积累的影响，发现甲基茉莉酸浓度对愈伤组织生物量及药效物质红景天苷积累有显著影响，其中25.0 mg/L MeJA对愈伤组织生物量及红景天苷的积累效果最好。因此，通过培养愈伤组织是一种获得大量红景天活性物质的有效方法，为红景天产品的生产提供一种原材料途径。
Abstract: A highly effective and rapid culture system of callus was developed by cultivating callus from leaves, stems and roots of Rhodiola L. The design and analysis of multivariate experiments showed the optimum medium for callus induction was MS + 2.0 mg/L 6-BA + 0.2 mg/L NAA + 0.1 mg/L 2,4-D. The effects of methyl jasmonate (MeJA) on callus biomass and bioaccumulation of salidroside were studied by adding MeJA. It was found that the concentration of MeJA had significant effects on callus biomass and bioaccumulation of salidroside, of which 25.0 mg/L MeJA had the best effect on callus biomass and accumulation of salidroside. Therefore, callus culture is an effective way to obtain a large number of active substances of Rhodiola, which provides a raw material for the production of Rhodiola products.
文章引用：张国珍, 谢洁, 秦小波, 牛蓓, 胡冬梅, 时小东, 樊莉娟, 徐心艺, 秦一菲. 红景天愈伤组织诱导及红景天苷积累的研究[J]. 植物学研究, 2019, 8(6): 438-444. https://doi.org/10.12677/BR.2019.86055

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