酶辅助水蒸气蒸馏提取迷迭香精油工艺的研究

Study on the Extraction of Rosemary Volatile Oil by Enzyme-Assisted Steam Distillation

摘要: 本文采用纤维素酶辅助的水蒸气蒸馏的提取方法，通过控制单因素变量法，探讨迷迭香中精油的最佳提取工艺。酶法辅助迷迭香精油的最佳提取工艺条件：加酶量为5 mg/g，酶解温度为30℃，酶解时间为3.5 h，料液比为1:8，水蒸馏时间为2.5 h，此时的提取率最高为1.357%。纤维素酶能够有效地破坏细胞壁，有利于迷迭香挥发油的释放。酶解条件温和，操作简便，有利于工业化生产。

Abstract: The optimum extraction process of volatile oil from rosemary was studied by cellulase assisted steam distillation using the single factor variable controlling method. The best enzymatic assisted extraction conditions of volatile oil from rosemary are the enzyme dosage of 5 mg/g, hydrolysis temperature of 30˚C, hydrolysis time of 3.5 h, ratio of material to liquid of 1:8, extraction time of 2.5 h, and the highest extraction rate of 1.357%. Cellulase can effectively destroy the cell wall and is beneficial to the release of volatile oil from rosemary. The hydrolysis conditions are mild, the operation is simple, and it is beneficial to industrial production.