丙泊酚联合脐带间充质干细胞上清液冻干粉对肝叶切除再生中炎症因子IL-10及IL-17的作用
The Effect of Propofol Combined with the Freeze Dried Powder of Umbilical Cord Mesenchymal Stem Cells on Interleukin10 and Interleukin17 in Partial Hepatectomy
DOI: 10.12677/ACM.2019.911208, PDF,    国家自然科学基金支持
作者: 李 娜：北京儿童医院保定医院五官科，河北 保定；孟慧鹏, 董化江, 王 平：天津大学精密仪器与光电子工程学院，天津；徐 健：武警后勤学院，天津
关键词: 丙泊酚；间充质干细胞；肝脏；白细胞介素；Diisopropylphenol； Mesenchymal Stem Cells； Liver； Interleukin
摘要: 目的：探讨丙泊酚联合脐带间充质干细胞上清冻干粉在部分肝切除术(PHT)后的大鼠中对白细胞介素-10 (interleukin-10, IL-10)及白细胞介素-17 (interleukin-17, IL-17)的影响。方法：将36只雄性Wistar大鼠随机分为3组，每组12只。A组麻醉用丙泊酚进行诱导和维持，打开胸腔暴露肝脏(假手术组)；B组的麻用丙泊酚加生理盐水干预进行诱导和维持，行部分肝切除术(PHT)；C组的麻醉用丙泊酚联合间充质干细胞上清液干预进行诱导和维持，行部分肝切除术(PHT)。在PHT术后第5天将大鼠处死，取出再生的肝脏，称重并评估。另外，将收集大鼠血清，用于测量IL-17和IL-10的水平。结果：在A组和C组之间，IL-17和IL-10水平没有差异，蛋白印迹检验结果显著升高(P > 0.05)；而C组IL-17的血浆水平跟组B相比明显降低(P < 0.05)，而IL-10明显升高(P < 0.05)。B和组C的平均肝再生率分别为19.45%、31.68%，差异显著，有统计学意义(P < 0.05)。结论：丙泊酚作为一种麻醉药物，可联合脐带间充质干细胞上清冻干粉，可有效降低促炎因子IL-17的表达，同时显著上调控炎因子IL-10的表达，促进残肝再生，可作为肝脏切除术后肝再生的重要手段。
Abstract: Objective: To investigate the effect of propofol combined with umbilical cord mesenchymal stem cell supernatant freeze drying powder and IL-10 and IL-17 after partial hepatectomy (PHT) in rats. Methods: 36 male Wistar rats were randomly divided into 3 groups with 12 rats in each group. Anesthesia was induced and maintained with propofol in group A, and the chest was opened to expose the liver (sham operation group). Anesthesia in group B was induced and maintained by propofol and saline intervention, and partial hepatectomy (PHT) was performed. Anesthesia in group C was induced and maintained with propofol combined with mesenchymal stem cell supernatant intervention and partial hepatectomy (PHT) was performed. Rat was executed five days after PHT. The regenerated liver was removed, weighed and evaluated. In addition, rat serum will be collected to measure levels of IL-17 and IL-10. Results: There was no difference in IL-17 and IL-10 levels between group A and group C (P > 0. 05). Compared with group B, plasma levels of IL-17 in group C were significantly decreased, while IL-10 in plasma and western blot was significantly increased (P < 0.05). The mean liver regeneration rate of group B and group C was 19.45% and 31.68%, respectively, with significant difference and statistical significance (P < 0.05). Conclusion: Propofol, as an anesthetic drug, can be used in combination with freeze-drying powder of umbilical cord mesenchymal stem cells, which can effectively reduce the expression of IL-17 and significantly up-regulate the expression of IL-10, promote liver regeneration, and can be used as an important means of liver regeneration after hepatectomy.
文章引用：李娜, 孟慧鹏, 董化江, 王平, 徐健. 丙泊酚联合脐带间充质干细胞上清液冻干粉对肝叶切除再生中炎症因子IL-10及IL-17的作用[J]. 临床医学进展, 2019, 9(11): 1346-1351. https://doi.org/10.12677/ACM.2019.911208

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