N-羟甲基酰胺类亲水抗菌剂的设计与合成

Design and Synthesis of N-Hydroxymethyl Amide-Based Antimicrobial Agents

摘要: 设计并合成了N-羟甲基甲酰胺(HOF)亲水抗菌剂，研究了枯草芽孢杆菌(Bacillus subtilis)和啤酒酵母(Saccharomyces cerivisiae)在含N-羟甲基甲酰胺的培养基中的培养特性，并进行了抑菌实验和核酸释放实验。结果表明，HOF能抑制枯草芽孢杆菌和啤酒酵母菌的生长，有显著的抗菌作用，且HOF浓度越高，抗菌效果越强。在同样条件下，HOF对枯草芽孢杆菌和啤酒酵母菌的抑菌作用强于甲酰胺。HOF对枯草芽孢杆菌细胞膜和细胞壁影响不大，但对啤酒酵母菌细胞壁和细胞膜有很强的破坏作用，导致细胞内核酸的外泄。

Abstract: N-hydroxymethyl formamide (HOF) was synthesized by reaction of formamide with formaldehyde in the presence of sodium hydroxide. HOF was tested against the Gram (+) bacteria Bacillus subtilis and the yeast Saccharomyces cerivisiae. HOF was active against the above bacteria and yeast, showing great potential as a new antimicrobial agent, since it was more active than formamide. After incubation of yeast Saccharomyces cerivisiae in HOF for 24 h, leakage of DNA from the cell was determined, indicating the considerable damage of cytoplasmic membrane. In the case of bacteria Bacillus subtilis, however, leakage of DNA from the cell was not observed.