超高压液相色谱法同时测定聚碳酸酯食品包装材料中五种酚类化合物的方法研究

Simultaneous Determination of Five Phenolic Compounds in Polycarbonate Food Packaging Materials by Ultra-High Performance Liquid Chromatography

摘要: 本文建立了超高圧液相色谱法同时测定聚碳酸酯食品包装材料中2-叔丁基苯酚等五种酚类化合物的方法研究。以甲醇为溶剂，分别制备五种酚类化合物标准曲线，过有机滤膜(0.22 μm)后分别采用FLD检测器和DAD检测器进行分析。结果表明，采用HPLC-FLD法，对叔丁基苯酚、2-叔丁基苯酚、4-叔辛基苯酚三种酚类在0.05 mg/L~5 mg/L范围内的线性良好，2,4-二叔丁基苯酚、4-肉桂苯酚两种酚类在0.1 mg/L~10 mg/L范围内的线性良好，五种酚类化合物的检出限为0.071 mg/L~0.371 mg/L，在5个加标水平下加标回收率为97.5%~102.3%，相对标准偏差为0.91%~1.1%。采用HPLC-DAD法，五种酚类化合物在1 mg/L~20 mg/L范围内的线性良好，五种酚类化合物的检出限为0.238 mg/L~0.477 mg/L，在20个加标水平下加标回收率为103.2%~111.7%，相对标准偏差为2.0%~2.6%。说明HPLC-FLD法比HPLC-DAD法表现出更高的灵敏度，这两种方法均可用于聚碳酸酯食品包装材料中五种酚类化合物的快速检测。

Abstract: This paper established a study on the simultaneous detection method of five phenolic compounds including 2-tert-butylphenol in polycarbonate food packaging materials by ultra-high pressure liquid chromatography. Standard curves of five phenolic compounds were prepared with methanol as solvent and analyzed by FLD detector and DAD detector after organic filter membrane (0.22 μm). The results showed that p-tert-butyl phenol, 2-tert-butylphenol and 4-tert-octylphenol had good linearity respectively in 0.05~5 mg/L by HPLC-FLD method, 2,4-tert-butylphenol and 4-cinnamon phenol had good linearity respectively in 0.1~10 mg/L by HPLC-FLD method. The detection limit of five phenolic compounds were in the range of 0.071~0.371 mg/L, the recovery in the 5 spiked level was 97.5%~102.3%, the relative standard deviation was 0.91%~1.1%. Five phenolic compounds had good linearity respectively in 1~20 mg/L by HPLC-DAD method, the detection limit of five phenolic compounds were in the range of 0.238~0.477 mg/L, the recovery in the 20 spiked level was 103.2%~111.7%, the relative standard deviation was 2.0%~2.6%. This indicated that HPLC-FLD method showed higher sensitivity than HPLC-DAD method. These two methods can be used for the rapid detection of five phenolic compounds in polycarbonate food packaging materials.