间充质干细胞治疗脑梗塞后脑脊液中血小板–内皮细胞黏附分子及Caspse-6的变化

The Change of the PECAM and Caspase-6 in Cerebrospinal Fluid of Cerebral Infarction (CI)Patients Treated with Umbilical Cord-Mesenchymal Stem Cells

摘要: 目的：探讨脐带华通氏胶源性间充质干细胞(umbilical cord mesenchymal stem cells, UC-MSCs)治疗脑梗塞的效果及对脑脊液内血小板–内皮细胞黏附分子(plate endothelial cell adhesion molecule-1, PECAM)及caspase-3的影响。方法：本研究共纳入颅脑创伤患者36人，入院时格拉斯哥评分无差异，随机分为常规治疗组和UC-MSCs治疗组，每组18人。对比以上两组患者CT或核磁的改变情况；对比两组患者的入院评分、住院时间和预后评分；脑脊液标本来源于临床生物样本库。本研究对脑脊液的中血小板–内皮细胞黏附分子(PECAM)及caspase-6的采用酶联免疫吸附试方法检测。结果：细胞治疗组患者 PECAM的表达高于对照组，差异显著，有统计学意义(P < 0.05)，细胞治疗组患者caspase-6表达低于对照组，差异显著，有统计学意义(P < 0.05)；UC-MSCs组住院时间及预后评分均优于对照组(P < 0.05)。结论：颅梗塞患者行常规治疗辅以UC-MSCs治疗既可有效上调PECAM的表达水平，同时又可有效地降低caspase-6的表达，对颅脑创伤患者住院时间和预后评分均有不同程度改善。

Abstract: Objective: To investigate the effect of umbilical cord mesenchymal stem cells (UC-MSCs) on immune microenvironment and angiogenesis reconstruction of cerebral infarction (CI). Methods: 36 cases of cerebrospinal fluid were divided into 2 groups, including control group and CI + UC-MSCs treatment group 18 cases in each group. PECAM and caspase-6 in cerebrospinal fluid were detected by Enzyme-linked immunosorbent assay (ELISSA) in each group. Results: PECAM expression in the cell treatment group was higher than that in the control group (P < 0.05), while caspase-6 expression in the cell treatment group was lower than that in the control group (P < 0.05), and the hospital stay and prognosis scores of the UC-MSCs group were better than those of the control group (P < 0.05). Conclusion: The main possible mechanism of UC-MSCs for treatment of CI was regulating stabilization of immune microenvironment and improving angiogenesis reconstruction in damaged brain tissue. Sequential treatment with mesenchymal stem cells of umbilical cord is an effective and safe treatment method for patients with craniocerebral trauma. UC-MSCs can effectively control inflammation and reduce the hospitalization time and prognosis score of patients.