腐殖酸对硫酸盐还原菌厌氧还原硫酸盐的抑制效果及其机理研究

The Role and Mechanism of Humic Acid in Inhibiting the Anaerobic Production of Sulfate by Sulfate Reducing Bacteria

摘要: 在含大量SRB的污泥中投加HA，在厌氧条件下进行摇瓶反应，结果表明：HA对SRB产生H₂S和硫化物有强烈的抑制效果，80~200 mg/L的HA在对产生H₂S的抑制效果超过80%，对硫化物的产生抑制效果超过50%；HA能够促进SRB对电子供体的利用，投加200 mg/L HA的SRB对电子供体的总利用率为97.88%，相比未投加HA的SRB提高了20.62%；投加AQDS的摇瓶实验间接验证了投加HA扰乱了SRB还原SO₄^2- 的电子传递体系，并成为SRB代谢的最终电子受体；HA对SRB代谢过程中的两种关键酶APS还原酶和SO₃^2- 还原酶都有显著的抑制作用，其抑制效果分别为44.59%和42.53%。

Abstract: The HA was added to sludge containing a large amount of SRB and reacted under anaerobic condi-tions. The results showed that HA strongly inhibited the production of H₂S and sulfides by SRB. The effect is that 80 to 200 mg/L of HA has an inhibitory effect on H₂S production of more than 80% and an inhibitory effect on sulfide production of more than 50%. HA can promote the use of SRB for electron donors. The total utilization of SRB for 200 mg/L HA for electron donors is 97.88%, which is 20.62% higher than that for SRB without HA. The addition of AQDS shake flask experiments indirectly verified that dosing HA disrupted the SRB-reduced SO₄^2- electron transport system and became the ultimate electron acceptor for SRB metabolism. HA has a significant inhibitory effect on two key enzymes in the metabolism of SRB:APS reductase and sulfite reductase, with inhibitory effects of 44.59% and 42.53%, respectively.