干旱及复水对皂角叶片生理特性及次生代谢的影响
Effects of Drought and Rehydration on Physiological Characteristics and Secondary Metabolism of Gleditsia sinensis Leaves
DOI: 10.12677/BR.2020.91009, PDF,    科研立项经费支持
作者: 康 瑞, 唐中华：东北林业大学化学化工与资源利用学院，黑龙江 哈尔滨；刘 佳：东北林业大学材料科学与工程学院，黑龙江 哈尔滨
关键词: 皂角；干旱；复水；生理；次生代谢；Gleditsia； Drought； Rehydration； Physiology； Secondary Metabolism
摘要: 为探讨皂角叶片对干旱胁迫及复水的生理响应。测定分析不同干旱胁迫程度及复水处理下，叶片的叶绿素含量、丙二醛含量、过氧化物酶活性等生理生化表征指标及酚类代谢的动态变化。结果显示，随干旱加剧，叶绿素含量降低；丙二醛含量、过氧化物酶活性升高；复水后各项指标均恢复到近似对照组水平，但与对照组有一定差距。此外，酚类代谢物在干旱及复水中产生了不同程度的变化。其中L-苯丙氨酸在干旱胁迫中含量升高，复水后含量降低；C6C3C6碳骨架的黄酮类化合物在干旱胁迫后含量降低，复水后略有恢复。证明在干旱中，皂角叶片启动了自身防御机制，抗氧化物酶和酚类化合物协同作用，减少活性氧对细胞的伤害。此外，皂角对于干旱胁迫的伤害具有一定的承受范围，高强度的胁迫会对皂角叶片造成不可逆的伤害。
Abstract: To study the physiological response of Gleditsia sinensis leaves to drought stress and rehydration, the changes of chlorophyll content, malondialdehyde content, peroxidase activity and phenols metabolism in leaves under different drought stress and rehydration were analyzed. The results showed that with the increase of drought, the content of chlorophyll decreased, the content of malondialdehyde and the activity of peroxidase increased, and all the indexes recovered to the approximate level of the control group after rehydrating, but there was a certain gap between the two groups. In addition, phenolic metabolites changed in different degrees in drought and rehydration. The content of L-phenylalanine increased in drought stress and decreased after rehydration; the content of flavonoids in C6C3C6 carbon skeleton decreased after drought stress and recovered slightly after rehydration. The results showed that the leaves of Gleditsia sinensis initiated the self defense mechanism, the antioxidant enzymes and phenols cooperated to reduce the damage of reactive oxygen species to cells. In addition, the damage of Gleditsia sinensis to drought stress has a certain range; high-intensity stress will cause irreversible damage to Gleditsia sinensis leaves.
文章引用：康瑞, 刘佳, 唐中华. 干旱及复水对皂角叶片生理特性及次生代谢的影响[J]. 植物学研究, 2020, 9(1): 65-75. https://doi.org/10.12677/BR.2020.91009

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