片仔癀对乳腺癌细胞凋亡的影响
Apoptosis Effect of Pien Tze Huang on Breast Cancer Cells
DOI: 10.12677/TCM.2020.92017, PDF,    科研立项经费支持
作者: 魏风琴：福建中医药大学附属第二人民医院急诊科，福建 福州；林小提：福建医科大学附属福建省妇幼保健院乳腺科，福建 福州
关键词: 片仔癀；乳腺癌；细胞凋亡；Pien Tze Huang； Breast Cancer； Cell Apoptosis
摘要: 目的：探讨片仔癀(Pien Tze Huang)对乳腺癌细胞(MDA-MB-231和MCF-7)凋亡的影响。方法：采用噻唑蓝/MTT测定细胞的存活率；使用流式细胞仪测定AnnexinV-FITC/PI细胞凋亡及细胞周期检测；应用Western Blot测定凋亡相关蛋白Bax和Bcl-2。结果：片仔癀对MDA-MB-231的细胞存活率呈时间和浓度相关性的显著性影响效果(p < 0.05)。AnnexinV-FITC/PI和细胞周期结果均表明，片仔癀可显著性地引起乳腺癌细胞的凋亡(p < 0.05)。同时，片仔癀可显著性地减少乳腺癌细胞中的抗凋亡蛋白Bcl-2的表达；相反地，片仔癀可显著性地增加乳腺癌细胞中的促进凋亡蛋白Bax的表达。结论：片仔癀可引起乳腺癌细胞凋亡，其在乳腺癌的治疗价值需要进一步探究。
Abstract: Objective: To investigate the apoptosis effect of Pien Tze Huang on breast cancer cells. Methods: MTT assay was performed to determine the cell viability of breast cancer cells. Further, both An-nexinV-FITC/PI expression and cell cycle of breast cancer cells were evaluated by flow cytometry. Moreover, apoptosis-related proteins, including Bcl-2 and Bax, were analyzed to evaluate. Results: MTT assay found that Pien Tze Huang induced apoptosis of breast cancer cells by a dose- and time-dependent manner. Both results of AnnexinV-FITC/PI and cell cycle further indicated that Pien Tze Huang resulted in significant apoptosis effect of breast cancer cells (p < 0.05). Furthermore, Pien Tze Huang significantly reduced Bcl-2 expression of breast cancer cells; on the contrary, Pien Tze Huang significantly increased Bax expression of breast cancer cells. Conclusion: Pien Tze Huang can result in the apoptosis effect of breast cancer cells. It is worth for investigators to further clarify the potential therapeutic application of Pien Tze Huang upon breast cancer.
文章引用：魏风琴, 林小提. 片仔癀对乳腺癌细胞凋亡的影响[J]. 中医学, 2020, 9(2): 108-114. https://doi.org/10.12677/TCM.2020.92017

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