生化方法检测毒力因子以诊断热不稳定肠毒素大肠杆菌导致的腹泻
Detection of Virulence Factor Delta by PCR and Biochemical Methods to Identify Heat-Labile Enterotoxigenic Escherichia coli-Caused Diarrhoea
摘要: 目的：探讨PCR及生物化学方法对确定ETEC的分子特性和毒力谱的重要意义，并通过检测毒力基因eltA的存在诊断热不稳定(LT)肠毒素产生的ETEC导致的腹泻。方法：采用RT-PCR方法，确认两株大肠杆菌为热不稳定的ETEC。被动胶乳凝集实验将被用来测试样品中LT毒素的存在。结果：在这项研究中，样本1和样本3均表达eltA基因，乳胶凝集显示样本3的呈阳性。结论：染色结果提示此腹泻儿童极有可能感染了热不稳定的ETEC。
Abstract: Objective: To investigate expressions and clinical significance of determining the molecular characteristic and identify the virulence profile of the ETEC and heat-labile (LT) enterotoxin producing ETEC can be identified by examining the presence of the virulence gene eltA. Methods: By using RT-PCR, two E. coli strains were confirmed to be the LT producing ETEC. The passive latex agglutination was allowed to test the presence of the LT toxin in the specimens. Results: In this study, both samples 1 and 3 specifically expressed the eltA gene, the sample 3 from child with diarrhoea was positive for the latex agglutination. Conclusion: It was highly likely that child was infected by LT producing ETEC stain.
文章引用：郑钰树. 生化方法检测毒力因子以诊断热不稳定肠毒素大肠杆菌导致的腹泻[J]. 医学诊断, 2020, 10(1): 20-25. https://doi.org/10.12677/MD.2020.101004

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