海洋红酵母ZOR1的鉴定及联合提取甘露聚糖和类胡萝卜素的研究

Identification of Marine Red Yeast ZOR1 and Extraction of Both Mannan and Carotenoids from ZOR1

摘要: 对PCR扩增的IST序列的分析比对及进化树的建立确定了一株从渤海湾水域分离的红酵母为海洋胶红酵母，命名为Rhodotorula mucilaginosa ZOR1 (R. mucilaginosa)；发酵培养R. mucilaginosa ZOR1后收集菌体，向菌体中加入不同浓度的蜗牛酶和蛋白酶进行破壁，并在破壁水相中提取甘露聚糖，细胞碎片中提取类胡萝卜素，结果表明当蜗牛酶和蛋白酶浓度分别为3%和0.2%时，甘露聚糖的提取率与对照相比提高10.36倍；提取甘露聚糖后的细胞碎片中继续加入0.6%的蛋白酶作用30 min，则类胡萝卜素的提取率可达到753 μg/g CDW，与对照相比提高10.76倍。

Abstract: A marine red yeast ZOR1 isolated from the Bohai Bay was identified by amplified IST sequence and designated as Rhodotorula mucilaginosa (R. mucilaginosa) ZOR1. Harvested R. mucilaginosa ZOR1 after cultivation was treated with snailase and proteinase to release both mannan and carotenoids. Then mannan and carotenoids were extracted from aqueous phase and cell fragments, respectively. The results showed that maximal yield of mannan was 10.36-fold that of the control when R. mucilaginosa ZOR1 cells were treated with 3% snailase and 0.2% proteinase. Carotenoids yield reached maximum about 753 μg/g CDW, which was 10.76-fold that of the control when the cell fragments of R. mucilaginosa ZOR1 treated with 3% snailase and 0.6% proteinase were hydrolyzed with 0.6% proteinase for another 30 min.