冷热型哮喘大鼠平滑肌内钙通道特性与差异

Characteristics and Differences of Calcium Channels in Smooth Muscle Cells of Hot and Cold Asthmatic Rats

摘要: 目的：检测冷热型大鼠支气管平滑肌细胞(ASMCs)内钙离子浓度，探讨两型支气管哮喘中的差异，为冷热型哮喘治疗提供理论依据。方法：27只SD大鼠随机分组为寒哮组、热哮组，正常对照组，每组9只，构建冷热哮喘模型后，应用共聚焦技术测定荧光值、荧光面积，以评估哮喘SD大鼠支气管平滑肌细胞钙离子浓度变化。结果：1) 荧光值比较：3组间差异，有统计学意义(P < 0.01)。3组间两两比较，差异均非常显著(P < 0.01)。热哮组 > 冷哮组 > 正常组。2) 荧光面积比较：3组间有统计学意义(P < 0.05)。3组间两两比较，热哮组 > 冷哮组 > 正常组。正常组与热哮组相比，有统计学意义(P < 0.05)，正常组与冷哮组、热哮组与冷哮组两两比较，差异无显著性(P > 0.05)。结论：支气管平滑肌钙离子浓度增加，是冷、热哮的共同特征，热哮组较冷哮组增加明显可能是热哮组的发病，多与感染相关，推测感染也是导致钙离子增高的一个因素。

Abstract: Objective: To detect the concentration of calcium ion in bronchial smooth muscle cells (ASMCs) of cold and hot asthmatic rats, to explore the differences between the two types of bronchial asthma, so as to provide a theoretical basis for the treatment of cold and hot asthma. Methods: Twenty-seven SD rats were randomly divided into three groups: the cold asthma group, the hot asthma group, and the normal control group, with 9 rats in each group. After establishing cold and hot asthma model, fluorescence value and fluorescence area were measured by confocal microscopy, to evaluate the changes of Calcium ion concentration in bronchial smooth muscle cells of asthmatic SD rats. Results: (1) Comparison of fluorescence values: there were significant differences between the three groups (P < 0.01). The pairwise comparison between the three groups was very significant (P < 0.01): The hot asthmatic rats group > the cold asthmatic rats group > the normal group. (2) Comparison of fluorescence area: the difference of fluorescence area between the three groups was statistically significant (P < 0.05): Comparison between the three groups, the hot asthmatic rats group > the cold asthmatic rats group > the normal group. There was statistical significance in the normal group and the hot asthmatic rats group (P < 0.05). There was no significant difference between the normal group and the cold asthma group, and between the hot asthma group and the cold asthma group (p > 0.05). Conclusion: The increase of calcium ion concentration in bronchial smooth muscle cells is a common feature of cold and hot asthma. The increase in the hot asthma group compared with the cold asthma group may obviously be the onset of the hot asthma group, which is mostly related to infection. It is speculated that infection is also a factor leading to increased calcium ions.