摘要: 目的：探讨糖尿病的发病机理，中药玉竹及舒糖宝对2型糖尿病动物模型的治疗效果的比较研究。方法：测定造模前后小白鼠血糖含量。然后用链佐霉素小剂量多次腹腔注射造模，每天1次，连续5天。造模后将小鼠分成正常组、模型组、治1组和治2组。治1组用玉竹治疗，治2组用舒糖宝治疗，模型组和正常组为不治疗对照组。结果：正常组、模型组、治1组、治2组依次为，模前血糖，6.18 ± 1.30、7.72 ± 1.55、7.61 ± 2.74、7.31 ± 1.36，各组比较，P > 0.05，差异无统计学意义；模后一周血糖，4.17 ± 1.30、7.02 ± 1.99、7.86 ± 2.92、6.89 ± 2.48；与正常组比较，P < 0.05，P < 0.01，差异有统计学意义；模后二周血糖，5.14 ± 1.03、11.52 ± 5.77、13.18 ± 6.87、11.26 ± 5.62；与正常组比较，P < 0.05，P < 0.01，差异有统计学意义；治后15天血糖分别是，5.02 ± 1.54、20.29 ± 5.81、21.65 ± 8.22、15.48 ± 5.91、与正常组比较，P < 0.01，差异有统计学意义；治后22天血糖，5.63 ± 1.00、25.50 ± 6.26、22.74 ± 6.48、23.37 ± 6.64；与正常组比较，P < 0.01，差异有统计学意义；治2组治后血糖有明显下降，其他组无明显变化。血清甘油三脂(TG)、总蛋白含量、超氧阴离子自由基(
)清除率(%)、浓度、尿糖含量、各组间差异有统计学意义。结论：用链佐霉素5次腹腔注射建立2型糖尿病模型成功，造模组造模后血糖比造模前明显升高，并明显高于正常组，造模成功；治2组治疗后血糖已见明显下降，血清甘油三脂(TG)较低，超氧阴离子自由基(
2)清除率(%)明显升高。
Abstract:
Objective: To investigate the pathogenesis of diabetes and to compare the therapeutic effect of the traditional Chinese medicine Yuzhu and Shutangbao on animal model of type 2 diabetes. Methods: The blood glucose of mice was measured before and after the model was made. Then a small dose of streptomycin was injected intraperitoneally for several times, once a day for 5 consecutive days. The mice were divided into normal group, model group, treatment group and treatment group. Treatment group 1 with Yuzhu treatment, treatment group 2 with Shutangbao treatment, model group and normal group as no treatment control group. Results: Premold blood glucose was 6.18 ± 1.30, 7.72 ± 1.55, 7.61 ± 2.74, and 7.31 ± 1.36 in the normal group, model group, treatment group 1 and treatment group 2, respectively. One week after mold, the blood glucose level was 4.17 ± 1.30, 7.02 ± 1.99, 7.86 ± 2.92, and 6.89 ± 2.48; compared with the normal group, P < 0.05, P < 0.01, the difference was statistically significant. Two weeks after the model, blood glucose was 5.14 ± 1.03, 11.52 ± 5.77, 13.18 ± 6.87, 11.26 ± 5.62; compared with the normal group, P < 0.05, P < 0.01, the difference was statistically significant. The blood glucose at 15 days after treatment was 5.02 ± 1.54, 20.29 ± 5.81, 21.65 ± 8.22, and 15.48 ± 5.91, respectively. Blood glucose at 22 days after treatment was 5.63 ± 1.00, 25.50 ± 6.26, 22.74 ± 6.48, and 23.37 ± 6.64, respectively; compared with the normal group (P < 0.01), and the difference was statistically significant. There was a significant decrease in blood glucose after treatment in 2 groups, but no significant change in other groups. Serum triglyceride (TG), total protein content, superoxide anion radical (
) clearance rate (%), concentration, urine sugar content, the differences between groups were statistically significant. Conclusion: The model of type 2 diabetes was successfully established by 5 intraperitoneal injections of streptomycin. The blood glucose of the model group was significantly higher than that of the normal group, and the model was successful. After treatment, the blood glucose in the 2 groups decreased significantly, the serum triglyceride (TG) was low and the superoxide anion radical (
) clearance rate (%) was significantly increased.