声光可调–近红外光谱技术即时快速检测川贝母中总生物碱含量方法研究

A Real-Time Rapid Analysis Method for the Determination of Total Alkaloids in Fritillariae Cirrhosae Bulbus by AOTF-NIR

摘要: 目的：建立一种采用声光可调–近红外光谱(AOTF-NIR)技术即时快速检测川贝母中总生物碱含量的新方法。方法：依据2015年版《中国药典》川贝母项下方法，测定川贝母中总生物碱含量，利用偏最小二乘法(PLS1)建立校正集样本总生物碱含量测定值与其NIR光谱间的定量分析模型，并对验证集样本进行预测。结果：建立的总生物碱定量分析模型准确性较好，外部交叉验证均方差(RMSEP)为0.0022，决定系数(R²)为0.9831；所建模型对验证集样品的验证结果较好，平均相对误差为2.16%；预侧值与化学测定值间相关系数r为0.9909。结论：该方法快速、准确、无污染，可用于川贝母中总生物碱含量的即时快速检测分析。

Abstract: Objective: To establish a new method for real-time rapid determination of total alkaloids in Fritil-lariae Cirrhosae Bulbus by acousto-optic tunable filter-near infrared (AOTF-NIR) spectroscopy. Methods: The content of total alkaloids in the sample was measured with the 2015 edition of Chinese Pharmacopoeia. Then, multivariate calibration models based on PLS1 algorithm were developed to correlate the spectra and the corresponding values determined by the reference methods. Results: The quantitative analysis models of total alkaloids content performed well. RMSEP of the model was 0.0022 and the correlation coefficient of the models was 0.9909. The mean relative error for the test sets was 2.16%. Conclusions: The method is rapid, accurate and pollution-free, and can be used for the rapid determination of total alkaloids in Fritillariae Cirrhosae Bulbus.