脐带间充质干细胞对高原脑水肿大鼠水通道蛋白-4的影响

The Effect of UC-MSCs on Aquaporins-4 Expression in High Altitude Cerebral Edema in Rats

摘要: [目的]探讨间充质干细胞对高原脑水肿大鼠水通道蛋白4表达的影响。[方法]成功制作大鼠高原缺氧损伤模型8只，对照组及干细胞组各8只，测脑组织含水量，采用RT-PCR、免疫组织化学、Western Blot测定脑组织高原缺氧损伤24 h后水通道蛋白4 mRNA和蛋白表达变及间充质干细胞对其影响。[结果]高原缺氧损伤后，脑组织含水量增加，水肿明显，水通道蛋白4 mRNA和蛋白表达量上调(P < 0.05)，应用间充质干细胞可有效抑制水通道蛋白4的表达。[结论]水通道蛋白4在高原缺氧脑水肿的形成过程中可能发挥了重要作用，应用间充质干细胞可有效抑制水通道蛋白4的表达，减轻高原脑水肿损伤。

Abstract: [Objective] To study the relationship between AQP4 and high altitude cerebral edema and effect of UC-MSCs. [Methods] The model of high altitude cerebral edema was established (N = 8). Brain edema was measured. The expression of AQP4 mRNA and protein were measured by RT-PCR, immunohistochemistry and Western Blot and to detect the effect of UC-MSCs in on Aquaporins-4 expression in high altitude cerebral edema in rats. [Results] The water content increased and the edema was evident. The expression of AQP4 mRNA and protein were up-regulated (P < 0.05) and UC-MSCs could inhibit the expression of AQP4 in mRNA and protein. [Conclusions] The expression of AQP4 mRNA and protein were up-regulated after hypoxia. UC-MSCs could inhibit the expression of AQP4 in mRNA and protein in high altitude cerebral edema.