不同浓度氯化钠胁迫对牵牛花种子发芽的影响

Effect of Sodium Chloride Stress with Different Concentration on Seed Germination of Pharbitis spp.

摘要: 以牵牛花种子为试材，研究了不同浓度氯化钠对牵牛花种子发芽特性的影响。结果表明：随着氯化钠浓度的升高，牵牛花种子的初始萌发时间与萌发高峰期均随之延迟；氯化钠浓度在50~200 mmol•L⁻¹范围内时，盐胁迫处理对牵牛花种子的发芽率影响不显著；当氯化钠浓度达到200 mmol•L⁻¹时，牵牛花种子的发芽势显著低于对照和其他盐胁迫处理，其他盐胁迫处理间的发芽势差异不显著，且与对照相比差异也都不显著；随着氯化钠浓度的升高，牵牛花种子的发芽指数逐渐降低，活力指数则是先升高后降低，发芽指数和活力指数除50 mmol•L⁻¹的氯化钠胁迫与对照差异不显著外，其他胁迫处理均显著低于对照；在50~200 mmol•L⁻¹的氯化钠范围内，各胁迫处理的盐害指数与对照差异不显著。试验结果表明，牵牛花种子可以耐200 mmol•L⁻¹的氯化钠胁迫，其种子发芽的耐盐极限浓度仍需进一步探索。

Abstract: The effect was studied about sodium chloride stress with different contents on seed germination of Pharbitis spp. The results showed that the initial germination time and the peak of germination of the seeds were delayed with the increase of the concentration of sodium chloride. The effect of salt stress on the germination rate of the seeds was not significant compared to control during the concentration of 50 - 200 mmol•L⁻¹; when 200 mmol•L⁻¹ sodium chloride solution, the germination vigor of the seeds was the lowest in the all treatments, and there was no significant difference in germination vigor between other salt stress treatments; other salt stress treatments were not sig-nificant difference compared with the control. The germination index decreased gradually, and the vigor index increased first and then decreased with the increase of the concentration of sodium chloride, and those of the seeds of other treatments except the germination index and vigor index of 50 mmol•L⁻¹ sodium chloride solution was lower than the control. In the concentration range of 50 - 200 mmol•L⁻¹, salt damage index was not significant compared to control. It indicated that Pharbitis spp. seed could withstand 200 mmol•L⁻¹ sodium chloride stress; and the salt tolerance limit concentration of seed germination still needs to be further explored.