肠道气泡堆积对银鲳四种组织代谢酶活力的影响
Effect of Intestinal Gas Bubble Accumulation on the Metabolizing Enzymes in Four Tissues of Silver Pomfret
DOI: 10.12677/OJFR.2020.72011, PDF,   
作者: 郑 迪：中国水产科学研究院东海水产研究所 农业农村部东海渔业资源开发利用重点实验室，上海;上海海洋大学水产与生命学院，上海；王 倩, 王 磊, 施兆鸿, 彭士明*：中国水产科学研究院东海水产研究所 农业农村部东海渔业资源开发利用重点实验室，上海
关键词: 银鲳；肠道气泡堆积；组织；代谢酶；Silver Pomfret； Intestinal Gas Bubble Accumulation； Tissue； Metabolizing Enzyme
摘要: 肠道气泡堆积是银鲳养殖过程中的常见病，具有发病快，死亡率高的特点，严重阻碍银鲳的规模养殖及推广。为研究肠道气泡堆积对银鲳组织代谢酶活力的影响，2019年3月于东海水产研究所福鼎研究中心采集了15尾肠道气泡堆积的银鲳为病鱼组(11.7 ± 0.8 cm, 42.8 ± 4.1 g)，15尾健康银鲳为对照组(健康鱼组) (12.5 ± 0.7 cm, 48.6 ± 5.6 g)，分别采集各组样品的肝脏，肾脏，肌肉和鳃组织，并对这四种组织谷草转氨酶活力(AST)、谷丙转氨酶活力(ALT)、碱性磷酸酶(ALP)和酸性磷酸酶活力(ACP)活力进行测定和比较，实验结果显示：病鱼组肌肉和肾脏中AST活力显著低于健康组(P < 0.05)；病鱼组肝脏和肌肉中的ALT活力显著低于健康组(P < 0.05)；两组样品四种组织中ALP活力均有显著差异；病鱼组肝脏中ACP活力显著高于健康组(P < 0.05)，但在鳃和肾脏中显著低于健康组(P < 0.05)。研究结果表明，肠道气泡堆积会导致银鲳组织代谢紊乱，且对银鲳肌肉ALT，肾脏ALP活力影响较大，这两种酶活力指标可以成为该病检测和病理分析的重要参考依据。
Abstract: Intestinal gas bubble accumulation is a common disease in the breeding process of silver pomfret, which is characterized by fast onset and high mortality, and seriously hinders the large-scale farm-ing and promotion of silver pomfret. In order to investigate the effect of intestinal gas bubble accu-mulation on the metabolizing enzyme activity of silver pomfret, fifteen diseased fish were chosen as group diseased (11.7 ± 0.8 cm, 42.8 ± 4.1 g) and fifteen healthy fish as group healthy (12.5 ± 0.7 cm, 48.6 ± 5.6 g). These fish were collected from Fuding research center of East China Sea Fisheries Re-search Institute in March 2019. Liver, kidney, muscle, and gill tissues of each group were collected and used to test their activities of AST, ALT, ALP, and ACP. The results showed that: the activities of in muscle and kidney in group diseased were significantly lower than those in group healthy (P < 0.05); the activities of ALT in liver and muscle in group diseased were significantly lower than those in group healthy (P < 0.05); the activities of ALP in four tissues of the two groups were significantly different; the activity of ACP in liver in group diseased were significantly lower than those in group healthy (P < 0.05), but in gill and kidney, it was significantly lower than those in group healthy (P < 0.05). The results showed that the intestinal gas bubble accumulation would lead to the disorder of tissue metabolism of silver pomfret, and had a great influence on the activity of ALT in muscle and ALP in kidney of silver pomfret. These two indexes of enzyme activity could be an important refer-ence for the detection and pathological analysis of the disease.
文章引用：郑迪, 王倩, 王磊, 施兆鸿, 彭士明. 肠道气泡堆积对银鲳四种组织代谢酶活力的影响[J]. 水产研究, 2020, 7(2): 77-83. https://doi.org/10.12677/OJFR.2020.72011

参考文献

[1] 彭士明, 尹飞, 孙鹏, 等. 不同饲料对银鲳幼鱼增重率、肝脏脂酶及抗氧化酶活力的影响[J]. 水产学报, 2010, 34(6): 769-774.
[2] 李建生, 胡芬, 严利平. 东海区银鲳资源合理利用的研究[J]. 自然资源学报, 2014, 29(8): 1420-1429.
[3] 张仁斋. 中国近海鱼卵与仔鱼[M]. 上海: 上海科学技术出版社, 1985: 151-153.
[4] 施兆鸿, 王建钢, 高露姣, 等. 银鲳繁殖生物学及人工繁育技术的研究进展[J]. 海洋渔业, 2005, 27(3): 246-250.
[5] 施兆鸿, 高露姣, 谢营梁, 等. 舟山渔场银鲳和灰鲳繁殖特性的比较[J]. 水产学报, 2006, 30(5): 647-653.
[6] 周燕侠, 余开. 这条鱼可以养殖了——揭开东海银鲳养殖的神秘面纱[J]. 科学养鱼, 2017(2): 13-16.
[7] Espmark, Å.M. and Baeverfjord, G. (2009) Effects of Hyperoxia on Behavioural and Physiological Variables in Farmed Atlantic Salmon (Salmo salar) Parr. Aquaculture International, 17, 341-353.
[Google Scholar] [CrossRef] 
[8] Edsall, D.A. and Smith, CE. (1990) Performance of Rainbow Trout and Snake River Cutthroat Trout Reared in Oxygen-Supersaturated Water. Aquaculture, 90, 251-259.
[Google Scholar] [CrossRef] 
[9] 彭天辉. 自然及人工条件下大口黑鲈慢性气泡病的病因及病理研究[D]: [硕士学位论文]. 上海: 上海海洋大学, 2013.
[10] 高权新, 谢明媚, 彭士明, 等. 急性温度胁迫对银鲳幼鱼代谢酶、离子酶活性及血清离子浓度的影响[J]. 南方水产科学, 2016, 12(2): 59-66.
[11] Kramer, D.L. (1987) Dissolved-Oxygen and Fish Behavior. Environmental Biology of Fishes, 18, 81-92.
[Google Scholar] [CrossRef] 
[12] Bouck, G.R. (1980) Etiology of Gas Bubble Disease. Transactions of the American Fisheries Society, 109, 703-707.
[Google Scholar] [CrossRef] 
[13] 李娟, 张耀庭, 曾伟, 等. 应用考马斯亮蓝法测定总蛋白含量[J]. 中国生物制品学杂志, 2000, 13(2): 118-120.
[14] 冯健, 刘永坚, 田丽霞, 等. 草鱼实验性镉中毒对肝胰脏、肾脏和骨骼的影响[J]. 水产学报, 2004, 28(2): 195-200.
[15] 王桂芹, 李子平, 牛小天, 等. 饲料能量和维生素B6对乌鳢生长和蛋白质代谢酶活性的影响[J]. 中山大学学报(自然科学版), 2011, 50(4): 96-99.
[16] Brãer, S. (2008) Amino Acid Transport across Mammalian Intestinal and Renal Epithelia. Physiological Re-views, 88, 249-286.
[Google Scholar] [CrossRef] [PubMed]
[17] 陈晨, 黄峰, 舒秋艳, 等. 共轭亚油酸对草鱼生长、肌肉成分、谷草转氨酶及谷丙转氨酶活性的影响[J]. 水生生物学报, 2010, 34(3): 647-651.
[18] 李丹丹, 陈丕茂, 朱爱意, 等. 黑鲷幼鱼力竭运动后代谢酶活性的恢复水平[J]. 南方水产科学, 2018, 14(6): 61-67.
[19] 安树伟, 袁思平, 王国良. 患诺卡氏菌病的大黄鱼几种主要组织的酶活力变化分析[J]. 渔业科学进展, 2012, 33(2): 43-48.
[20] 张永嘉, 吴泽阳, 许其爵, 等. 网箱养殖罗非鱼综合症的血清分析[J]. 水利渔业, 1994(2): 8-9+47.
[21] 刘巧巧. 中国大鲵肾脏与生殖腺发生的解剖学和组织学观察[D]: [硕士学位论文]. 西安: 陕西师范大学, 2012.
[22] 程波. 七带石斑鱼“突眼”症病原菌鉴定及其血液病理研究[D]: [硕士学位论文]. 乌鲁木齐: 新疆农业大学, 2009.
[23] 廖金花, 陈巧, 林丽蓉. 鲍鱼碱性磷酸酶的分离纯化和性质研究[J]. 厦门大学学报, 2005, 44(2): 272-275.
[24] 杜启艳, 王萍, 王友利, 等. 长期饥饿和再投喂对泥鳅不同组织糖原、酸性磷酸酶和碱性磷酸酶的影响[J]. 江西师范大学学报(自然科学版), 2008, 32(4): 114-119.
[25] 陈瑛, 邱子健, 隋淑光, 等. 棘尾虫酸性磷酸酶的定位及诱导表达[J]. 动物学报, 2003, 49(2): 218-223.
[26] Pipe, R.K. (1990) Hydrolytic Enzymes Associated with the Granular Haemocytes of the Marine Mussel My Tilus edulis. Histochemistry, 22, 596-603.
[Google Scholar] [CrossRef] 
[27] 江琰, 刘克武, 雷远成, 等. 意蜂工蜂酸性磷酸酶的纯化及其酶学特性[J]. 昆虫学报, 2004, 47(3): 310-315.
[28] 陈寅儿, 王国良, 金珊, 等. 三疣梭子蟹患“乳化病”后几种保护酶活力的变化[J]. 水产科学, 2006, 25(9): 448-451.

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