SLE患者外周血IFN-α、NE及MPO表达水平及意义

The Expression and Significance of Serum IFN-α, Serum NE and Serum MPO in Patients with Systemic Lupus Erythematosus

摘要: 目的：检测系统性红斑狼疮(SLE)患者血清干扰素α (IFN-α)、弹性蛋白酶(NE)及髓过氧化物酶(MPO)水平，探讨IFN-α、NE及MPO水平与SLE疾病活动度、血清学指标和临床表现的相关性及IFN-α、中性粒细胞胞外诱捕网(NETs)两者可能存在的关系。方法：采用酶联免疫吸附法(ELISA)测定SLE患者组与健康对照组血清IFN-α、NE及MPO水平，并分析上述指标之间及上述指标与疾病活动度、血清学指标和临床表现的相关性。结果：SLE患者组血清IFN-α、NE、MPO的水平较健康对照组显著增高(p < 0.05)，差异有统计学意义；SLE患者组血清IFN-α、NE、MPO的水平均与系统性红斑狼疮活动指数(SLEDAI)评分呈正相关(p < 0.05)；SLE患者组血清IFN-α水平与血清补体C3、血红蛋白(Hb)呈负相关(p < 0.05)，与红细胞沉降率(ESR)、24 h尿蛋白定量、抗双链DNA抗体(抗ds-DNA抗体)水平呈正相关(p < 0.05)，血清MPO水平与抗dsDNA抗体呈正相关(p < 0.05)；SLE患者组血清IFN-α水平与血液系统、泌尿系统、关节炎、皮疹、光过敏等临床表现呈正相关(p < 0.05)，血清NE水平与皮疹临床表现呈正相关(p < 0.05)；结果显示SLE患者组NE、MPO呈正相关(p < 0.05)，且IFN-α分别与NE、MPO呈正相关(p < 0.05)。结论：SLE患者组血清IFN-α、NE及MPO水平较健康对照组增高且与SLE中SLEDAI评分、部分血清学指标、临床表现相关，提示IFN-α、NETs水平在SLE发病中发挥作用。同时IFN-α分别与NE、MPO均呈正相关，说明IFN-α与NETs在SLE发病机理中可能具有相关性。

Abstract: Objective: To investigate the levels of serum interferon α (IFN-α), elastase (NE) and myeloperoxidase (MPO) in patients with systemic lupus erythematosus (SLE), to explore the correlation of IFN-α, NE and MPO levels with disease activity, serological indexes and clinical manifestations of SLE and the possible relationship between IFN-α and neutrophil extracellular traps (NETs). Methods: The levels of serum IFN-α, NE and MPO were measured by Enzyme-linked immunoassay (ELISA) method between SLE patients and healthy controls, to analyze the correlation of IFN-α, NE and MPO levels with disease activity, serological indexes and clinical manifestations in SLE patients. Results: The levels of IFN-α, NE and MPO in the SLE patient group were significantly higher than those in the healthy control group (p < 0.05); the levels of IFN-α, NE, MPO in patients with SLE were positively correlated with systemic lupus erythematosus disease activity index (SLEDAI) score; in patients with SLE, the level of serum IFN-α was negatively correlated with serum complement C3 and Hemoglobin (Hb) (p < 0.05), and positively correlated with erythrocyte sedimentation rate (ESR), 24-hour urinary protein and anti-dsDNA antibody level; the level of serum MPO in patients with SLE was positively correlated with anti-dsDNA antibody (p < 0.05); the level of serum IFN-α in patients with SLE was positively correlated with clinical manifestations such as blood system, urinary system, arthritis, rash, photosensitivity (p < 0.05); the level of serum NE in patients with SLE was positively correlated with the clinical manifestations of rash (p < 0.05). The results showed that NE and MPO in SLE patients were positively correlated (p < 0.05), and IFN-α was positively correlated with NE and MPO respectively (p < 0.05). Conclusion: The serum levels of IFN-α, NE and MPO in patients with SLE were higher than healthy control group and positively correlated with SLEDAI score, serological indexes, clinical manifestations, suggesting that the levels of IFN-α and NETs matter in the pathogenesis of SLE. IFN-α was positively correlated with NE and MPO respectively, indicating that IFN-α and NETs may be related in the pathogenesis of SLE.