茭白脱毒苗组织快繁技术研究
A Study on the Rapid Propagation Technology of Water-Free Virus Seeds
DOI: 10.12677/OJNS.2020.83025, PDF,    科研立项经费支持
作者: 柴飘飘, 陈昶旭, 褚晓洁, 查笑君*：浙江师范大学化学与生命科学学院，浙江 金华；陈晓荣, 杨梦飞, 李怡鹏, 张尚法：金华市农业科学研究院，浙江 金华
关键词: 茭白；茎尖；组织培养；Zizania latlfolia Turcz； Stem Tip； Tissue Culture
摘要: 通过不同生物激素含量和浓度的配比对脱毒苗茭白外植体组织培养的方法进行研究，确立了一套安全适宜的茭白离外植体组织再生培养体系，也为茭白苗人工培育和接种茭白菰黑粉菌提供一个良好的环境和试验材料。本文以茭白脱毒苗植株地下部分的芽和高约40 cm茭白脱毒苗的茎尖为外植体，在高约40 ms生根培养基的基础上通过添加NAA、6-BA、KT等激素及相应的生物素对外植体进行了诱导，并在基础上采取了一些外植体组织污染抗褐的方法，完成了对外植体消毒、芽的诱导、增殖和茭白生根外植体培养基中所含的植物激素和生长环境调节剂的筛选。在人工培育茭白外植体脱毒苗中，取得了一定的成效。
Abstract: Through the study of the tissue culture of T. chinensis explants with different hormone concentra-tion ratios, a suitable system for in vitro regeneration of T. chinensis has been established, and it also provides a good test material for artificial inoculation of T. glutinosa. In this paper, the shoots of the underground plant and the stem tip of the white seedlings about 40 cm in height were used as explants, and the corresponding biological hormones such as NAA, 6-BA, and KT were added on the basis of the MS culture medium, and some explant pollution was taken. The anti-brown method completes disinfection of explants, induction of buds, proliferation, and selection of plant growth regulators in the rooting medium. Some results have been achieved in cultivating the white virus-free seedlings.
文章引用：柴飘飘, 陈昶旭, 褚晓洁, 陈晓荣, 杨梦飞, 李怡鹏, 张尚法, 查笑君. 茭白脱毒苗组织快繁技术研究[J]. 自然科学, 2020, 8(3): 187-195. https://doi.org/10.12677/OJNS.2020.83025

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