短柄草植株高频再生及遗传转化体系的探索
The Exploration of High-Frequencies Plant Regeneration and Genetic Transformation System of Brachypodium distachyon
DOI: 10.12677/BR.2020.93030, PDF,    科研立项经费支持
作者: 宋昌梅：上海辰山植物园，中科院上海辰山植物科学研究中心，上海
关键词: 短柄草；幼胚；愈伤组织；农杆菌；遗传转化；Brachypodium distachyon； Immature Embryo； Callus； Agrobacterium； Genetic Transformation
摘要: 为了探究短柄草的快速繁殖体系，本文以短柄草为研究材料，利用组培技术，通过对短柄草的愈伤组织的诱导，建立起一套高效、重复性好的短柄草再生系统；同时对短柄草的遗传转化体系进行了探索。研究结果如下：(1) 选择种子作为短柄草的外植体，通过愈伤组织的诱导及分化，可以实现短柄草的高效再生；(2) 对农杆菌介导的短柄草幼胚愈伤组织遗传转化进行了探索，(3) 对短柄草幼胚愈伤组织进行了GUS染色。
Abstract: To explore the rapid propagation system of Brachypodium distachyon, a regeneration system of Bra-chypodium distachyon with high efficiency and good repeatability was established in this paper, using tissue culture techniques, and Brachypodium distachyon as research material with callus induction and differentiation. And the genetic transformation system of Brachypodium distachyon was explored. The results suggest that: (1) Immature embryos of Brachypodium distachyon were used as explants; then efficient plant regeneration of Brachypodium distachyon was achieved by callus induction and differentiation; (2) Genetic transformation system for Brachypodium distachyon immature embryo callus mediated by Agrobacterium was explored; (3) GUS staining was carried out on the immature embryo callus of Brachypodium distachyon.
文章引用：宋昌梅. 短柄草植株高频再生及遗传转化体系的探索[J]. 植物学研究, 2020, 9(3): 254-261. https://doi.org/10.12677/BR.2020.93030

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