基于生物信息学分析ENC1在胃肠道肿瘤中的表达及临床意义

The Expression and Clinical Significance of ENC1 in Gastrointestinal Tumors Were Analyzed Based on Bioinformatics

摘要: 目的：分析外胚层神经皮质基因1 (Ectodermal-neural cortex 1, ENC1)在胃肠道肿瘤中的表达情况及临床意义。方法：挖掘Oncomine及TCGA数据库中关于ENC1基因在胃癌及结直肠癌中的相关数据，进行ENC1表达水平与胃肠道肿瘤临床生物学特性的相关分析；利用数据库中生存数据进行生存分析；运用在线分析软件评价ENC1的表达与免疫浸润的相关性；运用String数据库构建与ENC1基因相互作用的蛋白网络。结果：ENC1在胃癌、结肠癌等多种癌症中表达上升，在中枢神经系统肿瘤中表达下调；高表达的ENC1与胃癌患者的不良预后呈正相关(OS HR = 1.49, 95%CI = 1.25~1.76, P = 4.3e−06; PFS HR = 1.39, 95%CI = 1.14~1.7, P = 0.0012)，与结肠癌患者的预后无关(OS HR = 0.81, P = 0.81; DFS HR = 0.88, P = 0.59)；进一步分析发现，ENC1的表达与多种免疫细胞浸润显著相关。结论：ENC1基因在胃肠道肿瘤中高表达，高表达的ENC1可能是胃癌预后不良的危险因素。

Abstract: Objective: Analysis of the expression and clinical significance of ENC1 in gastrointestinal tumors. Methods: Analyze the relevant data of ENC1 gene in gastric cancer and colorectal cancer in Oncomine and TCGA databases, and perform correlation analysis of ENC1 expression level and clinical biological characteristics of gastrointestinal tumors; use survival data in the database for survival analysis; use online analysis software to evaluate the correlation between ENC1 expression and immune infiltration; use the String database to construct a protein network that interacts with the ENC1 gene. Results: The expression of ENC1 was increased in gastric cancer, colon cancer and other cancers, and down-regulated in tumors of the central nervous system. The high expression of ENC1 was positively correlated with the poor prognosis of patients with gastric cancer (OS HR = 1.49, 95%CI = 1.25 - 1.76, P = 4.3e−06; PFS HR = 1.39, 95%CI = 1.14 - 1.7, P = 0.0012), and had nothing to do with the prognosis of patients with colon cancer (OS HR = 0.81, P = 0.81; DFS HR = 0.88, P = 0.59). The expression of ENC1 was significantly correlated with the infiltration of various immune cells. Conclusion: ENC1 gene is highly expressed in gastrointestinal tumors, and high expression of ENC1 may be a risk factor for poor prognosis of gastric cancer.