蒜头果中神经酸的高效液相色谱法检测

Determination of Nervonic Acid in Malania oleifera by High Performance Liquid Chromatography (HPLC)

摘要: 本文以蒜头果(Malania oleifera)种子为研究材料，通过高相液相色谱法建立一种快速测定蒜头果种子中神经酸含量的方法。采用Aglient C-18 ODS柱(250 mm × 4.6 mm, 5 μm)色谱柱，以甲醇:乙腈:四氢呋喃:0.4%乙酸水溶液(体积比为10:80:5:5)为流动相，检测波长为205 nm，流速1.0 mL/min，柱温25℃，进样量10 μL。结果显示蒜头果中神经酸的浓度在1 mg/mL~10 mg/mL范围内与峰面积呈现良好的线性关系，相关系数R = 0.9984，并且精密度、稳定性、重复性的RSD均<3.0%，平均加样回收率为98.2%，RSD为1.36%。表明该方法准确，重复性好，能够适用于蒜头果中神经酸含量的快速检测。

Abstract: A method for the rapid determination of the content of nervonic acid in the seeds of Malania oleifera was established by High Performance Liquid Chromatography (HPLC). Nervonic acid was separated by Agilent C-18 ODS column (250 mm × 4.6 mm, 5 μm), using methanol:acetonitrile:tetra- hydrofuran:0.4% acetic acid (volume ratio 10:80:5:5) was used as mobile phase, the detection wavelength was 205 nm, the flow rate was 1.0 mL/min, the column temperature was 25℃, and the injection volume was10 μL. The results show that the concentration of nervonic acid in the range of 1 mg/mL - 10 mg/mL showed a good linear relationship with the peak area, and the correlation coefficient was R = 0.9984, and the RSD of precision, stability and repeatability were all < 3.0%, the average recovery rate was 98.2%, and the RSD was 1.36%. The method is accurate and reproducible, and can be used for rapid determination of the content of nervonic acid in the seeds of M. oleifera.