氯硝柳胺乙醇胺联合埃克替尼体外对肺腺癌HCC827细胞的协同抗肿瘤效应
In Vitro Synergistic Antitumor Effect of Niclosamide Ethanolamine Combined with Icotinib on HCC827 Cell
DOI: 10.12677/ACM.2020.107186, PDF,   
作者: 张 君, 王红梅：青岛大学附属医院呼吸内科，山东 青岛
关键词: 氯硝柳胺乙醇胺；盐酸埃克替尼；协同抗肿瘤；Chloronitramine Ethanolamine； Icotinib； Synergistic Antitumor
摘要: 目的：明确氯硝柳胺乙醇胺与盐酸埃克替尼联合应用在体外对人肺腺癌HCC827细胞的增殖抑制作用，探索两药合用是否具有协同抗肿瘤效应。方法：确定盐酸埃克替尼的半数抑制浓度、氯硝柳胺乙醇胺的半数抑制浓度，根据已确定的氯硝柳胺乙醇胺的IC50固定三个浓度，考察HCC827细胞增殖受到的抑制情况。绘制两药联合对HCC827细胞增殖的抑制曲线，检测两药是否有协同效应。结果：盐酸埃克替尼对HCC827细胞的IC50为0.61 μM。氯硝柳胺乙醇胺对HCC827细胞的IC50为0.46 μM。两药联用时，氯硝柳胺乙醇胺1.0 μM的处理组对细胞的抑制作用最明显，在极低浓度下细胞的生长即可被明显抑制。盐酸埃克替尼、氯硝柳胺均可以促进HCC827的细胞凋亡。联合应用组与单独应用组相比，HCC827细胞的晚期凋亡和坏死的比例都有所增加。结论：氯硝柳胺乙醇胺对人肺癌HCC827细胞株的增殖具有抑制作用，氯硝柳胺乙醇胺联合盐酸埃克替尼对人肺癌HCC827细胞有协同促进凋亡的作用。
Abstract: Objective: To investigate the inhibitory effect of clonsalimine ethanolamine and ectinib hydrochloride on HCC827 cells in human lung adenocarcinoma in vitro and explore whether the combination of the two drugs has a synergistic anti-tumor effect. Methods: The half inhibitory concentration of ectinib hydrochloride and the half inhibitory concentration of niclosamine ethanolamine were determined, and the inhibition of HCC827 cell proliferation was investigated according to the fixed IC50 concentration of niclosamine ethanolamine. The inhibition curve of the combination of two drugs on HCC827 cell proliferation was drawn to detect the synergistic effect of the two drugs. Results: The IC50 of ectinib hydrochloride on HCC827 cells was 0.61 M. The IC50 of niclosamide ethanolamine against HCC827 cells was 0.46 M. When the two drugs were used together, the inhibition of cell growth was the most obvious in the treatment group of 1 M of niclosamide ethanolamine, and the growth of cells was significantly inhibited at very low concentration. Both ectinib hydrochloride and niclosamine could promote apoptosis of HCC827 cells. The ratio of late apoptosis and necrosis of HCC827 cells was increased in the combined group compared with the single group. Conclusion: Clonsalimine ethanolamine can inhibit the proliferation of HCC827 cell lines in human lung cancer, and clonsalimine ethanolamine combined with ectinib hydrochloride can promote the apoptosis of HCC827 cell lines in human lung cancer.
文章引用：张君, 王红梅. 氯硝柳胺乙醇胺联合埃克替尼体外对肺腺癌HCC827细胞的协同抗肿瘤效应[J]. 临床医学进展, 2020, 10(7): 1211-1220. https://doi.org/10.12677/ACM.2020.107186

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