人工培育的蝉花子实体对H22荷瘤小鼠免疫功能影响的研究

Effects of Artificial Cultivated Cordyceps cicadae on Immune Function of H22 Tumor-Bearing Mice

摘要: 目的：本试验主要研究人工培育的蝉花虫草子实体对H22荷瘤小鼠免疫功能的影响。方法：40只ICR小鼠在腋下通过皮下接种H22肝癌小鼠腹水建立荷瘤小鼠的模型，接瘤24 h后进行随机分组，模型组、蝉花虫草子实体低、中、高剂量组、环磷酰胺阳性对照组，每组8只小鼠，共40只。蝉花虫草子实体低、中、高剂量组的小鼠分别按照75、150、450 mg/kg体重的剂量灌服蝉花虫草子实体生理盐水混悬液，每天1次；阳性对照组的小鼠按照20 mg/kg体重的剂量隔天腹腔注射环磷酰胺生理盐水溶液。连续给药14 d后，测各组小鼠的瘤体积、瘤重、肿瘤抑制率、脾脏指数、胸腺指数、血清免疫球蛋白和细胞因子含量、脾T淋巴细胞增殖能力。结果：各剂量组小鼠的瘤体积和瘤重均极显著低于模型组(P < 0.01)，四组小鼠的肿瘤抑制率分别为49.13%、55.72%、56.53%、62.59%；低、中剂量组小鼠脾脏指数和胸腺指数均高于模型组(P < 0.05)，高剂量组小鼠胸腺指数高于模型组(P < 0.05)，低剂量组OD值大于模型组(P < 0.05)；中、高剂量组和阳性对照组小鼠血清中IgA、IgG、IgM、IL-2、IL-6和TNF-α含量均高于模型组(P< 0.05)。结论：人工培育的蝉花虫草子实体通过调节小鼠的免疫功能抑制肿瘤的生长。

Abstract: Objective: To study the effect of artificial cultured Cordyceps cicadae on immune function of H22 tumor-bearing mice. Methods: 40 ICR mice were randomly divided into groups after 24 h of tumor-bearing mice, 8 mice in each group with low, medium and high dose group and cyclophosphamide positive control group. At 75, 150, 450 mg/kg body weight, the mice in the low, medium and high dose groups were fed with the suspension of physiological saline of Cordyceps cicadae fruit, each 1 day; mice in the positive control group were intraperitoneally injected with cyclophosphamide saline solution every other day at a dose of 20 mg/kg body weight. The tumor volume, tumor weight, tumor inhibition rate, spleen index, thymus index, serum immunoglobulin and cytokine content, spleen T lymphocyte proliferation ability of mice in each group were measured after 14 d of continuous administration. Results: The tumor volume and tumor weight of mice in each dose group were significantly lower than those in the model group (P < 0.01). The tumor inhibition rate in the four groups was 49.13%, 55.72%, 56.53%, 62.59%, respectively. The gland index was higher than that of the model group (P < 0.05), the thymus index of the mice in the high dose group was higher than that in the model group (P < 0.05), and the OD value in the low dose group was higher than that in the model group (P < 0.05). Conclusion: Artificially cultivated Cordyceps cicadae can inhibit tumor growth by regulating immune function in mice.