Mir-135a-3p在无功能垂体腺瘤中的表达及对其侵袭性的影响

Expression of MiR-135a-3p in Non-Functional Pituitary Adenomas and Its Effect on Its Invasiveness

摘要: 目的：本文探讨miR-135a-3p在无功能垂体腺瘤(NFPA)中的表达及其对NFPA细胞侵袭性的影响。方法：采用实时定量PCR分别检测50例的NFPA组织(侵袭性和非侵袭性各25例)；培育正常大鼠垂体瘤细胞株，随机分为实验组和阴性对照组，分别转染miR-135a-3p inhibitor和miR-135a-3p NC，采用实时荧光定量PCR检测miR-135a-3p的表达；转染后分别进行CCK8实验检测垂体瘤细胞增殖能力；Transwell实验检测垂体瘤细胞的迁移能力。结果：结果显示，侵袭性NFPA组中miR-135a-3p表达量明显高于非侵袭性NFPA组(P < 0.05)；转染miR-135a-3p inhibitor后垂体瘤细胞株的细胞增殖能力较阴性对照组下降(P < 0.05)；miR-135a-3p inhibitor组垂体瘤细胞与阴性对照组相比细胞迁移能力下降(P < 0.05)。结论：miR-135a-3p在NFPA中过表达，其可能调控垂体瘤细胞的增殖从而促进其侵袭行为的发生。

Abstract: Objective: To investigate the expression of miR-135a-3p in nonfunctional pituitary adenoma (NFPA) and its effect on the invasion of NFPA cells. Methods: 50 NFPA tissues (25 invasive and 25 non-invasive) were detected by real-time quantitative PCR. Normal rat pituitary tumor cell lines were cultured and randomly divided into the experimental group and the negative control group. miR-135a-3p inhibitor and miR-135a-3P NC were transfected respectively, and the expression of miR-135a-3P was detected by quantitative real-time PCR. CCK8 assay was used to detect the proliferation of pituitary tumor cells after transfection. Transwell assay was used to detect the migration ability of pituitary tumor cells. Results: The expression level of miR-135a-3p in the invasive NFPA group was significantly higher than that in the non-invasive NFPA group (P < 0.05). After transfection with miR-135a-3p inhibitor, the proliferation ability of pituitary tumor cell lines decreased compared with the negative control group (P < 0.05). Cell migration was decreased in the miR-135a-3p inhibitor group compared with the negative control group (P < 0.05). Conclusion: Overexpression of miR-135a-3p in NFPA may regulate the proliferation of pituitary tumor cells and promote their invasion.