利用响应面法摸索薄皮甜瓜采后病害生防菌株发酵最佳培养基

Using Response Surface Methodology to Explore the Best Fermentation Medium for Biocontrol Strain for Post-Harvest Diseases of Melon

摘要: 目的：研究对薄皮甜瓜采后病害具有防治作用的生防菌株浅黄假单胞菌的实验室发酵最佳培养基，为微生物农药应用开发提供优质资源。方法：采用Design-Expert 8.0软件的Plackett-Burman及Box- Behnken设计试验。结果：确定了对引起薄皮甜瓜采后病害主要病原真菌具有拮抗作用的生防菌株浅黄假单孢菌(Pseudomonas lutea)实验室发酵的最佳培养基为：葡萄糖20 g/L，MgSO₄ 0.2 g/L，牛肉膏8.3 g/L，酵母膏6.9 g/L，K₂HPO₄ 1 g/L，CaCO₃ 1 g/L。

Abstract: Aim: To study the best culture medium for laboratory fermentation of the biocontrol strain Pseudomonas lutea, which has a preventive effect on the postharvest diseases of muskmelon, and then provide high-quality resources for the application and development of microbial pesticides. Methods: Plackett-Burman and Box-Behnken design experiments using Design-Expert 8.0 software. Results: The optimal medium for laboratory fermentation of the biocontrol strain Pseudomonas lutea, which has antagonistic effect on the main pathogenic fungi that cause the postharvest diseases of muskmelon, was determined as: Glucose 20 g/L, MgSO₄ 0.2 g/L, beef extract 8.3 g/L, yeast extract 6.9 g/L, K₂HPO₄ 1 g/L, CaCO₃ 1 g/L.