黄芩素在烟曲霉菌性角膜炎中的抗炎作用

Anti-Inflammatory Effect of Baicalein in Aspergillus Fumigatus Keratitis

摘要: 黄芩素(Baicalein, BE)是从双子叶唇形科植物黄芩(Scutellaria baicalensis)的干燥根中提取分离出来的一种黄酮类天然小分子化合物。研究发现黄芩素在感染性疾病中具有抗真菌，抗炎，抗氧化，调节机体免疫功能等作用。但在真菌性角膜炎中尚未有研究，本文就黄芩素在烟曲霉菌性小鼠角膜炎模型中的作用进行研究。方法：体外实验用烟曲霉菌感染HCECs后，再用黄芩素处理，PCR和ELISA评估IL-1β，TNF-α，IL-6的mRNA及蛋白的表达。体内实验用烟曲霉菌菌丝感染C57BL/6小鼠角膜制造烟曲霉菌角膜炎模型，再用黄芩素或DMSO处理，PCR和ELISA评估IL-1β，TNF-α，IL-6的mRNA及蛋白的表达。结果：黄芩素通过降低IL-1β，TNF-α，IL-6mRNA及蛋白减轻角膜炎的炎症反应。结论：黄芩素通过抑制促炎因子表达减轻小鼠角膜炎炎症反应。

Abstract: Baicalein (BE) is a natural small molecular compound of flavonoids extracted from the dried roots of Scutellaria baicalensis, a plant in the family of dicotyledons and labiatae. Studies have found that BE has anti-fungal, anti-inflammatory, anti-oxidant, and immune function regulation effects in infectious diseases. But it has not been studied in fungal keratitis. This article studies the role of BE in the mouse model of Aspergillus fumigatus (A. fumigatus) keratitis. Methods: In vitro experiments. After HCECs were infected with A. fumigatus, they were treated with BE, PCR and ELISA were used to evaluate the mRNA and protein expression of IL-1β, TNF-α, and IL-6. In vivo experiments, the cornea of C57BL/6 mice was infected with A. fumigatus hyphae to create an A. fumigatus keratitis model, and then it was treated with BE or DMSO. PCR and ELISA evaluated the mRNA and protein expression of IL-1β, TNF-α, IL-6. Results: BE reduced the inflammatory response of keratitis by reducing IL-1β, TNF-α, IL-6mRNA and protein. Conclusion: BE reduces the inflammatory response of mouse keratitis by inhibiting the expression of pro-inflammatory factors.