多肽纳米盘抑制乳腺癌迁移的体外研究

Inhibitory Effect of Peptide Nanodiscs on Breast Cancer Migration In Vitro

摘要: 目的：乳腺癌发生转移是患者死亡的主要原因。我们前期研发了具有良好抑制肿瘤细胞迁移的多肽。然而多肽在体内降解快，不稳定，影响药效。因此，亟需一种方法来降低多肽降解速率。方法：采用脂质纳米盘包裹多肽可以保护多肽，提高多肽稳定性。结果：本文成功构建了多肽纳米盘，纳米盘粒径为16.36 ± 0.37 nm，PDI为0.387 ± 0.039，粒径均匀。多肽的包封率为80.86% ± 0.77%，载药量为1.43% ± 0.01%。多肽对MDA-MB-231细胞没有细胞杀伤作用，空白纳米盘对MDA-MB-231细胞有显著杀伤效果，IC50为262.6 ug/ml。多肽能够显著抑制MDA-MB-231细胞的细胞迁移，在一定浓度范围，多肽浓度越高，抑制作用越强。结论：多肽纳米盘在体外能够显著抑制乳腺癌细胞转移，为转移性乳腺癌治疗提供新方法。

Abstract: Objective: Breast cancer metastasis is the main cause of death. We have developed a peptide with good inhibition of tumor cell migration. However, the degradation of peptides in vivo is fast and unstable, which affects the efficacy of drugs. Therefore, there is an urgent need for a method to reduce the degradation rate of peptides. Methods: The peptide coated with lipid nanoparticles can protect the peptide and improve the stability of the peptide. Results: In this paper, peptide nanodiscs were successfully constructed. The diameter of nanodiscs was 16.36 ± 0.37 nm, and the PDI was 0.387 ± 0.039. The encapsulation efficiency was 80.86% ± 0.77%, and the drug loading was 1.43% ± 0.01%. The peptide had no cytotoxic effect on MDA-MB-231 cells, while blank nanodiscs had a significant killing effect on MDA-MB-231 cells with IC50 of 262.6 ug/ml. The polypeptide could significantly inhibit the migration of MDA-MB-231 cells. In a certain concentration range, the higher the peptide concentration, the stronger the inhibition effect. Conclusion: Peptide can significantly inhibit the migration of breast cancer cells in vitro, which provides a new method for the treatment of metastatic breast cancer.