微小RNA-744在动脉粥样硬化患者中表达的诊断和预后意义评估

Diagnostic and Prognostic Significance of microRNA-744 Expression in Patients with Atherosclerosis

摘要: 目的：动脉粥样硬化(Atherosclerosis, AS)是一种常见的血管疾病，是冠心病、缺血性心肌病、心力衰竭、血栓栓塞病的病理基础，具有较高的发病率和死亡率。近期大量的研究证明microRNAs (miRNAs)在AS的诊断和预后中发挥着至关重要的作用。本研究通过检测miR-744在AS中的表达情况，进一步评估miR-744对AS诊断和预后价值的影响。方法：采用荧光定量PCR技术检测120例AS患者和58例健康对照人群血清中miR-744的表达情况。通过受试者工作特征曲线(ROC曲线)分析miR-744的诊断的价值。使用Spearman相关系数评估miR-744与颈动脉内膜–中膜厚度(CIMT)的相关性，利用Kaplan-Meier生存曲线和多因素Cox回归分析miR-744在AS中的预后价值。结果：相较于健康对照组，AS患者血清中miR-744的表达水平显著降低(P < 0.001)，且miR-744的表达水平与CIMT值呈显著负相关性(r = −0.776, P < 0.001)。ROC曲线的AUC为0.887，特异性为93.33%，敏感性为82.85%，证实miR-744可能是AS的潜在的良好诊断标志物。生存分析表明miR-744低表达的患者发生心血管事件的概率较高(log rank P = 0.005)，miR-744可能是AS的潜在预后标志物(HR = 2.680, 95%CI = 1.105~6.503, P = 0.029)。结论：miR-744的低表达可能是良好的AS诊断标志物，并且与AS的不良预后相关。

Abstract: Objective: Atherosclerosis (AS) is a common vascular disease that is the pathological basis of coronary heart disease, ischemic cardiomyopathy, heart failure, thromboembolic disease, and has high morbidity and mortality. A large number of recent studies have demonstrated that microRNAs (miRNAs) play a crucial role in the diagnosis and prognosis of AS. The aim of this study was to examine the expression level of miR-744 in AS and to evaluate the diagnostic and prognostic value of miR-744 in AS. Methods: The expression of miR-744 in serum of AS patients and healthy control group was detected by fluorescence quantitative PCR. The diagnostic value of miR-744 was analyzed by the ROC curve. Spearman correlation coefficient was used to evaluate the correlation between miR-744 and carotid intima-media thickness (CIMT), and Kaplan-Meier survival curve and Multivariate Cox regression were used to analyze the prognostic value of miR-744 in AS. Results: Compared with the healthy control group, the expression level of miR-744 in the serum of AS patients was significantly reduced (P < 0.001), and the expression level of miR-744 was negatively correlated with the CIMT value (r = −0.776, P < 0.001). miR-744 is confirmed to be a potential good diagnostic marker for AS. The AUC of ROC curve is 0.887, the specificity is 93.33%, and the sensitivity is 82.85%. Survival analysis showed that low expression of miR-744 predicted a higher probability of cardiovascular events (log rank P = 0.005), and miR-744 might be a potential prognostic marker of AS (HR = 2.680, 95%CI = 1.105 - 6.503, P = 0.029). Conclusion: The low expression of miR-744 is a good diagnostic marker for AS, and is related to the poor prognosis of AS.