血清miR-223预测多发性骨折患者术后感染的临床价值分析

Clinical Value Analysis of Serum miR-223 in Predicting Postoperative Infection of Multiple Fractures Patients

摘要: 目的：本研究旨在探讨多发性骨折术后感染患者中血清miR-223的表达水平，并对其诊断价值进行评估。方法：选取于我院收治的120例多发性骨折患者，根据术后感染情况，分为感染组(n = 65)和非感染组(n = 55)。通过qRT-PCR对患者术前血清miR-223及C-反应蛋白(CRP)的表达水平进行检测。通过绘制受试者工作特征曲线对其诊断价值进行评估。结果：多发性骨折术后感染组患者血清miR-223表达水平显著低于非感染组。血清miR-223预测多发性骨折术后感染的受试者工作特征曲线下面积(AUC)为0.822，灵敏度和特异度分别为84.6%和74.5%。血清CRP预测多发性骨折患者术后感染的AUC为0.812，灵敏度和特异度分别为76.9%和72.7%。血清miR-223和CRP联合诊断的AUC为0.928，敏感度(96.4%)和特异度(76.9%)均高于单一指标的诊断结果。结论：血清miR-223在多发性骨折术后感染的患者中低表达，可能是预测多发性骨折术后感染的一个潜在的生物标志物。血清miR-223和CRP联合诊断能够提高诊断的准确性。

Abstract: Aim: This study aimed to investigate the expression levels of miR-223 in the serum of multiple fractures patients with postoperative infection, and assess its diagnostic value. Methods: 120 multiple fractures patients were selected, and divided into infection group (n = 65) and non-infection group (n = 55). Serum levels of miR-223 and C-reactive protein (CRP) were detected by using qRT-PCR. The receiver operating characteristics (ROC) curves were plotted to evaluate the diagnostic value. Results: Multiple fractures patients in postoperative infection group had high levels of miR-223 than those in non-infection group. The areas under receiver operating characteristics curves (AUC) of serum miR-223 in predicting infection after reoperation was 0.822, with the sensitivity of 84.6% and specificity of 74.5%. The AUC of serum CRP in predicting infection after reoperation was 0.812, with the sensitivity of 76.9% and specificity of 72.7%. The AUC of serum miR-223 combined with CRP reached up to 0.928, with the sensitivity of 96.4% and the specificity of 76.9%, which were higher than that of the diagnostic value of a single index. Conclusion: miR-223 was low-expressed in the serum of multiple fracture patients with postoperative infections, and might be a promising biomarker for predicting postoperative infection after multiple fractures. The combined detection of serum miR-223 and CRP might improve the diagnostic efficacy.