miR-1294在胃癌中表达的临床意义及其对胃癌细胞增殖、侵袭和迁移的影响

Clinical Significance of miR-1294 Expression in Gastric Cancer and Its Effect on Proliferation, Invasion and Migration of Gastric Cancer Cells

摘要: 目的：探讨miR-1294在胃癌中表达的预后价值，及其对胃癌细胞增殖、侵袭和迁移的影响。方法：通过实时荧光定量PCR (qRT-PCR)检测miR-1294在胃癌组织及癌旁正常组织中的表达情况，分析其与患者临床病理特征的关系，并利用Kaplan-Meier生存曲线和Cox回归分析对其在胃癌中的预后价值进行评估。在胃癌细胞中，通过MTT和Transwell实验检测miR-1294表达量对癌细胞生命活动的影响。结果：胃癌组织中miR-1294的表达水平显著低于癌旁正常组织(P < 0.05)，且与肿瘤组织分化程度、淋巴结转移及TNM分期均显著相关(P < 0.05)。miR-1294低表达患者的总体生存率较差(log-rank P < 0.01)。多因素Cox分析表明，miR-1294是胃癌的独立预后因子。MTT和Transwell检测显示，胃癌细胞增殖、侵袭和迁移能力在miR-1294干扰组中较对照组显著增加(P < 0.01)。结论：低表达miR-1294促进胃癌细胞的增殖、侵袭和迁移，与胃癌的不良预后相关。

Abstract: Aims: To explore the prognostic value of miR-1294 expression in gastric cancer patients and investigate the effect of miR-1294 on cancer cell proliferation, invasion and migration. Method: The expression of miR-1294 was detected by quantitative real-time PCR (qRT-PCR), and the relevance of miR-1294 to the clinicopathological feature was further analyzed. The prognostic value of miR-1294 was evaluated using Kaplan-Meier survival analysis and Cox regression assay. Effects of miR-1294 expression on cell biological behaviors of gastric cancer cells were analyzed using MTT and Transwell analysis. Results: The miR-1294 expression in gastric cancer tissues was significantly lower than that in the adjacent normal ones (P < 0.05), which was significantly related to the tumor differentiation, lymph node metastases and TNM stage (P < 0.05). The gastric cancer patients with lower miR-1294 expression had poor overall survival compared with those with higher miR-1294 expression (log-rank P < 0.01). According to the Cox analysis, the miR-1294 expression was identified as an independent prognostic factor for gastric cancer. MTT and Transwell assay showed that the cell proliferation, invasion and migration in the miR-1294 interference group were significantly promoted compared with corresponding controls (P < 0.01). Conclusion: Down regulated miR-1294 expression promotes the proliferation, invasion and migration of gastric cancer cells, which is associated with poor prognosis of gastric cancer.