松郁安神方对失眠大鼠基底核腺苷受体与多巴胺受体的影响
Effects of Songyu Anshen Prescription on Basal Adenosine Receptor and Dopamine Receptor in Insomnia Rats
DOI: 10.12677/TCM.2021.101006, PDF,    国家自然科学基金支持
作者: 王秀峰, 张 瑜, 曾雪爱, 张一帆, 陈铭奇, 黄俊山*：福建省中医药科学院，福建省中医睡眠医学重点实验室，黄俊山福建省名老中医药专家传承工作室， 福建 福州
关键词: 松郁安神方；失眠；腺苷受体；多巴胺受体；Songyu Anshen Prescription； Insomnia； Adenosine Receptors； Dopamine Receptor
摘要: 目的：观察中药复方松郁安神方(SYF)对失眠大鼠基底核腺苷受体和多巴胺受体的调节作用。方法：利用失眠大鼠模型，造模成功后分别给予SYF高剂量、SYF低剂量和阿普唑仑用药1周。治疗过程中观察实验大鼠的一般情况变化。治疗结束后，运用Western Blotting实验检测基底核组织腺苷A2AR受体和多巴胺受体D2DR蛋白表达水平。结果：对照组大鼠一般生理状况正常，模型组大鼠失眠引起的症状明显，SYF高剂量组和低剂量组大鼠失眠引起的症状显著改善。同对照组相比较，模型组基底核组织中A2AR蛋白表达水平被显著下调(P < 0.01)，D2DR蛋白表达水平被显著上调(P < 0.01)。而阿普唑仑组、SYF高剂量组和低剂量组则使得失眠大鼠基底核组织中A2AR蛋白表达水平被显著上调(P < 0.01)，D2DR蛋白表达水平被显著下调(P < 0.01)。结论：SYF可以改善实验大鼠因失眠引起的症状，其机制可能与调控失眠大鼠基底核腺苷受体和多巴胺受体的表达相关。
Abstract: Objective: To observe the regulatory effects of the traditional Chinese medicine Songyu Anshen prescription (SYF) on basal adenosine and dopamine receptors in insomnia rats. Methods: Using the insomnia rat model, SYF high dose, SYF low dose and Alprazolam were administered for 1 week after successful modeling. The general changes of experimental rats were observed during the treatment. After treatment, the protein expression levels of adenosine A2AR receptor and do-pamine receptor D2DR in basal ganglia were detected by Western Blotting experiment. Results: The rats in the control group generally had normal physiological conditions, and the symptoms caused by insomnia in the model group were obvious, while the symptoms caused by insomnia in the SYF high-dose group and the low-dose group were significantly improved. Compared with the control group, the expression level of A2AR protein in the basal ganglia of the model group was significantly down-regulated (P < 0.01); the expression level of D2DR was significantly up-regulated (P < 0.01). However, the Alprazolam group, the SYF high dose group and the low dose group significantly up-regulated the expression level of A2AR protein in basal ganglia of insomnia rats (P < 0.01); the expression level of D2DR was significantly down-regulated (P < 0.01). Conclusion: SYF can improve the symptoms caused by insomnia in experimental rats, and its mechanism may be related to the regulation of the expression of basal adenosine receptor and dopamine receptor in insomnia rats.
文章引用：王秀峰, 张瑜, 曾雪爱, 张一帆, 陈铭奇, 黄俊山. 松郁安神方对失眠大鼠基底核腺苷受体与多巴胺受体的影响[J]. 中医学, 2021, 10(1): 52-59. https://doi.org/10.12677/TCM.2021.101006

参考文献

[1] 张娅, 黄俊山, 曾雪爱, 张一帆. 失眠当重肝郁[J]. 福建中医药大学学报, 2013, 23(2): 61-62.
[2] 曾雪爱, 周春权, 张一帆, 王秀峰, 张瑜, 黄俊山. 松郁安神方对对氯苯丙氨酸致失眠大鼠血浆中前列腺素D2的影响[J]. 世界睡眠医学杂志, 2020, 7(1): 9-10.
[3] 张一帆, 李星, 张瑜, 李璟怡, 王秀峰, 曾雪爱, 张娅, 黄俊山. 松郁安神方对肝郁证失眠大鼠机体褪黑素水平的影响[J]. 世界睡眠医学杂志, 2020, 7(3): 548-551.
[4] 曾雪爱, 周春权, 王秀峰, 张一帆, 黄俊山. 松郁安神方对异相睡眠剥夺大鼠海马CREB表达的影响[J]. 福建中医药, 2018, 49(2): 14-16.
[5] 李璟怡, 黄俊山, 陈沁, 魏敏, 王海生, 陈铭奇. 松郁安神方对肝郁失眠大鼠行为学及5-HT1A受体基因表达的影响[J]. 福建中医药, 2019, 50(6): 22-24, 29.
[6] 李璟怡, 黄俊山, 陈沁, 王秀峰, 张瑜, 张一帆. 松郁安神方对失眠大鼠海马cAMP/PKA信号通路的影响[J]. 北京中医药大学学报, 2020, 43(3): 212-217.
[7] 肖成荣, 马增春, 李海静, 谭洪玲, 梁乾德, 王宇光, 陆倍倍, 高月. PCPA失眠大鼠模型的制作及其机制[J]. 毒理学杂志, 2007, 21(4): 326.
[8] 王秀峰, 李星, 张瑜, 曾雪爱, 张一帆, 黄俊山. 疏肝安神法防治失眠与多巴胺受体途径的相关性研究[J]. 中医学, 2019, 8(2): 74-80.
[9] 赵忠新. 临床睡眠障碍学[M]. 上海: 第二军医大学出版社, 2003: 26-27.
[10] 黄志力. 腺苷和多巴胺受体调控睡眠觉醒的作用与机制[J]. 中国药理学与毒理学杂志, 2018, 32(9): 737.
[11] 周娇娇, 陈旭, 王刚. 作用于腺苷受体改善失眠药物的研究进展[J]. 中国医刊, 2019, 54(1): 24-26.
[12] Lazarus, M., Shen, H.Y., Cherasse, Y., Qu, W.M., Huang, Z.L., Bass, C.E., Winsky-Sommerer, R., Semba, K., Fredholm, B.B., Boison, D., Hayaishi, O., Urade, Y. and Chen, J.F. (2011) Arousal Effect of Caffeine Depends on Adenosine A2A Receptors in the Shell of the Nucleus Accumbens. Journal of Neuroscience, 31, 10067-10075. [Google Scholar] [CrossRef] 
[13] Yuan, X.S., Wang, L., Dong, H., Qu, W.M., Yang, S.R., Cherasse, Y., Lazarus, M., Schiffmann, S.N., d’Exaerde, A.D.K., Li, R.X. and Huang, Z.L. (2017) Striatal Adenosine A2A Receptor Neurons Control Active-Period Sleep via Parvalbumin Neurons in External Globus Pallidus. eLife, 6, e29055. [Google Scholar] [CrossRef] 
[14] 刘炜, 黄志力, 曲卫敏. 腺苷A2A受体激动剂CGS21680模拟前列腺素D2诱发大鼠生理性睡眠[J]. 中国药学杂志, 2009, 44(23): 1786-1790.
[15] 许奇, 曲卫敏, 黄志力. 多巴胺D2受体调控睡眠–觉醒研究进展[J]. 中国临床药理学与治疗学, 2010, 15(4): 361-366.
[16] Qu, W.M., Xu, X.H., Yan, M.M., Wang, Y.Q., Urade, Y. and Huang, Z.L. (2010) Essential Role of Dopamine D2 Receptor in the Maintenance of Wakefulness, but Not in Homeostatic Regulation of Sleep, in Mice. Journal of Neuroscience, 30, 4382-4389. [Google Scholar] [CrossRef]