HPLC法测定胃疡宁丸中去甲乌药碱的含量
Determination of Higenamine in Weiyangning Pills by HPLC
DOI: 10.12677/OJNS.2021.91007, PDF,   
作者: 潘殿玲：济南市妇幼保健院，山东 济南；张海峰*：山东爱维德生物科技有限公司，山东 济南
关键词: 胃疡宁丸；去甲乌药碱；高效液相法；Weiyangning Pills； Higenamine； HPLC
摘要: 目的：建立一种胃疡宁丸中去甲乌药碱含量的高效液相色谱(HPLC)测定方法。方法：色谱柱选用安捷伦ZORBAX Eclipse XDB-C18液相色谱柱；流动相为乙腈–水(85:15)，水相中含有0.1%的甲酸；检测波长为214 nm，柱温25℃，流速为0.5 mL/min。结果：该方法具有较高的精密度、稳定性和可重复性，加样回收率平均值为99.33%，选取不同批次的胃疡宁丸，测定其中去甲乌药碱的平均含量为9.348 μg/g。结论：该方法可作为检测胃疡宁丸中去甲乌药碱含量的有效方法。
Abstract: Objective: To establish an effective method for determining Higenamine in Weiyangning pills by HPLC. Methods: ZORBAX Eclipse XDB-C18 was used as the column; the mobile phase was acetonitrile-water (85:15) with 0.1% of formic acid in water; the detection wavelength was 214 nm; the temperature was 25˚C; the flow rate was 0.5 mL/min. Results: The detection method exhibited high precision, stability, and repeatability. The average recovery was 99.33%. The average content of Higenamine in different batches of Weiyangning pills was 9.348 μg/g. Conclusion: This method can be used as an effective method for determining Higenamine in Weiyangning pills.
文章引用：潘殿玲, 张海峰. HPLC法测定胃疡宁丸中去甲乌药碱的含量[J]. 自然科学, 2021, 9(1): 48-53. https://doi.org/10.12677/OJNS.2021.91007

参考文献

[1] 中华人民共和国卫生部. WS3-B-3935-98. 中药成方制剂药品标准. 胃疡宁丸[S]. 北京: 中华人民共和国卫生部药典委员会, 1998.
[2] 何风雷, 郑华珠, 石洪超, 等. HPLC法测定胃疡宁丸中芍药苷的含量[J]. 中国医药导报, 2017, 14(9): 133-135.
[3] 黄思明, 梁志健, 操红缨, 等. 胃疡宁丸抗炎镇痛的药效研究[J]. 中药与临床, 2015(2): 64-67.
[4] 许艺飞, 江伟雯, 邝兆进, 等. 胃疡宁丸体内外抗幽门螺杆菌的实验研究[J]. 中药新药与临床药理, 2014(5): 572-576.
[5] 刘静, 黄萍, 吴清和, 等. 胃疡宁丸对胃肠运动影响的研究[J]. 中国实验方剂学杂志, 2013(10): 283-285.
[6] 徐晶晶, 黄萍, 吴清和, 等. 胃疡宁丸抗实验性胃溃疡的药效及机制研究[J]. 中国中药杂志, 2013, 38(5): 736-739.
[7] 李诗慧, 何广俊, 卢映, 等. HPLC法测定胃疡宁丸中乌药醚内酯的含量[J].中国医药指南, 2015, 13(6): 32-33.
[8] 邢梦雨, 田崇梅, 夏道宗. 乌药化学成分及药理作用研究进展[J]. 天然产物研究与开发, 2017, 29(12): 153-157.
[9] 韦震鸣, 傅强, 李志樑, 等. 去甲乌药碱对2型心肾综合征大鼠心肾的保护作用[J]. 实用医学杂志, 2018, 34(11): 1830-1833.
[10] Yang, S., Chu, S., Ai, Q., et al. (2020) Anti-Inflammatory Effects of Higenamine (Hig) on LPS-Activated Mouse Microglia (BV2) through NF-κB and Nrf2/HO-1 Signaling Pathways. International Immunopharmacology, 85, Article ID: 106629. [Google Scholar] [CrossRef] [PubMed]
[11] Romeo, I., Parise, A., Galano, A., et al. (2020) The Antioxidant Capability of Higenamine: Insights from Theory. Antioxidants, 9, 358. [Google Scholar] [CrossRef] [PubMed]
[12] Yen, C.-C., Tung, C.-W., Chang, C.-W., et al. (2020) Potential Risk of Higenamine Misuse in Sports: Evaluation of Lotus Plumule Extract Products and a Human Study. Nutrients, 12, 285. [Google Scholar] [CrossRef] [PubMed]
[13] Zhang, Y., Zhang, J., Wu, C., et al. (2019) Higenamine Protects Neuronal Cells from Oxygen-Glucose Deprivation/Reoxygenation-Induced Injury. Journal of Cellular Biochemistry, 120, 3757-3764. [Google Scholar] [CrossRef] [PubMed]
[14] Wang, Y., Geng, J., Jiang, M., et al. (2019) The Cardiac Electrophysiology Effects of Higenamine in Guinea Pig Heart. Biomedicine & Pharmacotherapy, 109, 2348-2356. [Google Scholar] [CrossRef] [PubMed]
[15] 曹艳, 王自正, 王峰, 等. 盐酸去甲乌药碱心肌负荷试验对心率、血压、心肌耗氧量的影响[J]. 中国医院药学杂志, 2012(17): 1353-1355.
[16] 宋莉, 贾琦, 周银银, 等. 基于生物大数据库分析并验证附子去甲乌药碱抗肿瘤潜在作用机制[J]. 南京中医药大学学报, 2020(5): 655-660.