珍稀植物掌叶木繁育试验

Breeding Experiment of Rare Plant Palm Leaf Tree

摘要: 采用离体培养法，以掌叶木[Handeliodendron bodinieri (Lev1.) Rehd.]种子为外植体，在含有不同植物激素的培养基上诱导萌芽及丛芽。结果表明，在适量的生长调节剂作用下，掌叶木种子培养发芽率可达95%，最佳诱导培养基为MS + 6-BA 1.0 mg/L + α-NAA 0.5 mg/L，最佳增殖培养基为1/2 MS + 6-BA 0.5 mg/L + NAA 0.25 mg/L。采用采用B5培养基代替MS培养基，且将大量元素降为1/2，能有效抑制愈伤组织的玻璃化。

Abstract: In vitro culture was used to induce germination and cluster buds on the medium containing dif-ferent plant hormones by using the seeds of Handeliodendron bodinieri (Lev1.) Rehd. as explants. The results showed that: the optimum medium for seed germination is supplied with MS + 6-BA 1.0 mg/L + α-NAA 0.5 mg/L; the germination rate is 95%; the optimum medium for bud differentiation is supplied with 1/2 MS + 6-BA 0.5 mg/L + NAA 0.25 mg/L. And, the vitrification of callus could be effectively inhibited by using B5 medium instead of MS medium and reducing the amount of elements to 1/2.