底栖硅藻悬浮培养初探

Preliminary Study on Suspension Culture of Benthic Diatoms

摘要: 底栖硅藻是鲍鱼和海参的主要饵料，足量优质饵料是人工育苗生产中最为重要因素，底栖硅藻大部分采用固定化培养和静置培养，无法达到规模生产，因此其工业化大规模生产十分必要。本文以舟形藻为研究材料，研究了不同硅浓度静置培养、不同硅浓度悬浮培养、光合反应器培养三种培养方式，打破传统的附着性生长培养状态，通过悬浮培养达到较高的培养密度。通过其OD值和细胞密度比较分析，初步得出对舟形藻生长影响最佳培养条件和最佳硅浓度，结果表明，悬浮条件培养下，最有利于舟形藻培养，藻细胞密度最高可达2.45 × 10⁶个/mL，为大规模悬浮培养优质舟形藻提供初步参考。

Abstract: Benthic diatoms are the main food for abalone and sea cucumber. Sufficient and high-quality food is the most important factor in the production of artificial seedlings. Most benthic diatoms are cultured in immobilized culture and static culture, which cannot achieve large-scale production, therefore, its large-scale industrial production is very necessary. In this paper, three kinds of culture methods, I. E. Static culture with different silicon concentration, suspension culture with different silicon concentration and photosynthetic reactor culture are studied, which broke the traditional adherent growth culture state and achieved high culture density through suspension culture. The optimum culture conditions and the optimum silicon concentration were obtained by comparing the OD value and the cell density. The results showed that the suspension culture was the most favorable for the cultivation of Navicular, and the maximum cell density was 2.45 × 10⁶ cells/mL, which provided a preliminary reference for large-scale suspension culture of high quality Navicular.