人参皂苷Rb1对糖尿病大鼠心脏结构功能和心肌纤维化的影响

The Effects of Ginsenoside Rb1 on Cardiac Structure, Function and Myocardial Fibrosis in Diabetes Rats

摘要: 目的：探究人参皂苷Rb1对糖尿病雄性大鼠心脏结构与功能和心肌纤维化的作用。方法：将24只大鼠随机分为对照组、糖尿病组、糖尿病 + 10 mg/kg Rb1组和糖尿病 + 20 mg/kg Rb1组，通过腹腔注射链脲佐菌素(streptozotocin, STZ)构建糖尿病大鼠模型。糖尿病 + 10 mg/kg Rb1组和糖尿病 + 20 mg/kg Rb1组每天分别腹腔注射10 mg/kg与20 mg/kg的Rb1，持续8周。8周后麻醉大鼠，采用超声心动图测量左室结构和功能参数，测血清血脂、肿瘤坏死因子-α (tumor necrosis factor-α, TNF-α)、白介素-6 (interleukin-6, IL-6)和脑钠肽(brain natriuretic peptide, BNP)水平。取大鼠心脏测量左心室重量指数(left ventricular weight index, LVWI)、单位质量心肌组织中羟脯氨酸与胶原蛋白含量、心肌胶原容积分数(collagen volume fraction, CVF)。结果：STZ处理后大鼠血糖均高于16.7 mmol/L，模型构建成功。与对照组相比，糖尿病大鼠组心脏结构和功能参数无显著性改变，而血清TNF-α、IL-6、BNP水平显著性升高，而20 mg/kg人参皂苷Rb1能够降低TNF-α、IL-6和BNP水平，差异有显著性意义(P < 0.05)。糖尿病大鼠LVWI、单位质量心肌组织中羟脯酸与胶原蛋白含量、心肌中CVF显著性增高(P < 0.05)。人参皂苷Rb1能显著降低LVWI、单位质量心肌组织中羟脯氨酸与胶原蛋白含量、心肌中CVF。结论：人参皂苷Rb1具有减轻炎症反应和心肌纤维化、延缓糖尿病心肌病发生发展的作用。

Abstract: AIM: To investigate the effects of ginsenoside Rb1 on cardiac structure, function and myocardial fibrosis in diabetes rats. METHODS: 24 rats were randomly divided into 4 groups: the control group, the diabetes group, the diabetes + 10 mg/kg Rb1 group and the diabetes + 20 mg/kg Rb1 group. The diabetes rats model was constructed by intraperitoneal injection of streptozotocin (STZ). Intraperitoneal injections of 10 mg/kg and 20 mg/kg Rb1 were administered daily for 8 weeks in the diabetes + 10 mg/kg group and diabetes + 20 mg/kg Rb1 group, respectively. Left ventricular structure and function were measured by echocardiography, and tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), brain natriuretic peptide (BNP) were measured by ELISA. Left ventricular weight index (LVWI), hydroxyproline and collagen content per unit mass in myocardial tissue, and collagen volume fraction (CVF) in myocardium were measured. RESULTS: After intraperitoneal injection of STZ, the blood glucose of rats was higher than 16.7 mmol/L, and the diabetes model was constructed successfully. Compared with the control group, cardiac structure and function parameters did not changed significantly, but serum TNF-α, IL-6 and BNP levels were higher in the diabetes rat groups, and Ginsenoside Rb1 20 mg/kg can reduce their levels significantly (P < 0.05). LVWI, hydroxyproline and collagen content per unit mass myocardial tissue, CVF in myocardium of diabetes rats were increased significantly (P < 0.05). Ginsenoside Rb1 significantly reduced LVWI, hydroxyproline acid and collagen content per unit mass in myocardial tissue, and CVF in myocardium (P < 0.05). CONCLUSION: Ginsenoside Rb1 can alleviate the inflammation and myocardial fibrosis of diabetes rats, and delay the occurrence and development of diabetic cardiomyopathy.