MiR-320a作为阿尔茨海默氏病的诊断生物标志物，对β淀粉样蛋白诱导的神经毒性具有保护作用
MiR-320a as a Diagnostic Biomarker for Alzheimer’s Disease and Its Protective Role against Amyloid-β Induced Neurotoxicity
DOI: 10.12677/ACM.2021.112094, PDF,    科研立项经费支持
作者: 张 群*, 钟月红, 沈 菊：宝山区老年护理院，上海；侯莉芳：宝山区庙行镇社区卫生服务中心，上海
关键词: MiR-320a；阿尔茨海默症；诊断；神经毒性；PTEN；MiR-320a； Alzheimer’s Disease； Diagnosis； Neurotoxicity； PTEN
摘要: 目的：MicroRNA (miRNA)在包括阿尔兹海默症(AD)在内的多种人类疾病中异常表达。本研究分析了miR-320a在AD患者中的表达变化和临床价值，并进一步研究了miR-320a在Aβ诱导的SH-SY5Y细胞神经毒性中的作用。方法：qRT-PCR用于测量miR-320a表达水平。建立ROC曲线以评估miR-320a对AD的诊断潜力。CCK-8和流式细胞仪用于细胞活力和凋亡的检测。荧光素酶报告基因测定进行靶基因确定。结果：AD患者的血清miR-320a低于健康对照组，并且与患者的MMSE评分呈正相关。血清miR-320a可以将AD患者与对照组区分开，在0.827的临界值下，诊断敏感性和特异性分别为85.5%和87.0%。MiR-320a减弱了Aβ25-35对SH-SY5Y细胞的神经毒性，而PTEN是被证明是其靶基因。结论：本结果为miR-320a作为诊断AD的有前途的生物标志物提供了依据，miR-320a的过表达通过靶向PTEN减弱了Aβ诱导的神经毒性。
Abstract: Objective: A large amount of microRNAs (miRNAs) have been found to be abnormally expressed in various human diseases, including Alzheimer’s disease (AD). The current study investigated the expression change and clinical value of miR-320a in AD patients, and further examined the role of miR-320a in Aβ-induced neurotoxicity in SH-SY5Y cells. Methods: qRT-PCR was used for the measurement of miR-320a expression levels. ROC curve was established to calculate the diagnostic potential of miR-320a for AD. CCK-8 and flow cytometry assay was applied for the detection of cell viability and apoptosis. Luciferase reporter assay was performed to confirm whether PTEN was the target gene of miR-320a. Results: Serum miR-320a was lower in AD patients than that in healthy controls, and was positively associated with the MMSE score in patients with AD. Serum miR-320a had the potential to distinguish AD patients from controls, and the diagnostic sensitivity and specificity were respectively 85.5% and 87.0% at a cutoff value of 0.827. MiR-320a attenuated Aβ25–35 induced neurotoxicity in SH-SY5Y cells, and PTEN was the target gene. Conclusion: The present results provide evidence for miR-320a as promising biomarker for the diagnosis of AD, and overexpression of miR-320a attenuated Aβ induced neurotoxicity in AD by targeting PTEN.
文章引用：张群, 侯莉芳, 钟月红, 沈菊. MiR-320a作为阿尔茨海默氏病的诊断生物标志物，对β淀粉样蛋白诱导的神经毒性具有保护作用[J]. 临床医学进展, 2021, 11(2): 646-654. https://doi.org/10.12677/ACM.2021.112094

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