清热解毒方与软坚散结方对人肺腺癌A549细胞增殖及Bcl-2表达的影响

Effects of Qingrejiedu Formula and Ruanjiansanjie Formula on Proliferation and Bcl-2 Expression of A549 Cells

摘要: 目的：研究清热解毒方与软坚散结方对人肺腺癌A549细胞增殖及Bcl-2表达的影响。方法：将不同浓度的清热解毒方、软坚散结方分别作用于人肺腺癌A549细胞，CCK8法检测细胞生长抑制率，流式细胞仪检测细胞凋亡率，RT-PCR检测Bcl-2 mRNA表达。结果：清热解毒方、软坚散结方均呈剂量依赖性抑制A549细胞生长(P < 0.05)，并下调Bcl-2 mRNA表达(P < 0.05)。结论：清热解毒方、软坚散结方均能不同程度地抑制人肺腺癌A549细胞的增殖，其机制可能与下调Bcl-2 mRNA表达有关，从而促进人肺腺癌A549细胞凋亡。

Abstract: Objective: To study the effects of Qingrejiedu Formula and Ruanjiansanjie Formula on proliferation and Bcl-2 expression of human lung adenocarcinoma cancer cell A549. Methods: We added different concentrations of Qingrejiedu Formula and Ruanjiansanjie Formula to human lung adenocarcinoma cells A549 and CCK8 method was used to detect cellular proliferation. Flow cytometric was used to detect cell apoptotic. RT-PCR method was used to detect the mRNA expression of Bcl-2. Results: Qingrejiedu Formula and Ruanjiansanjie Formula inhibited the proliferation of A549 cells in a dose-dependent manner (P < 0.05), and down-regulated the expression of Bcl-2 (P < 0.05). Conclusion: Qingrejiedu Formula and Ruanjiansanjie Formula can inhibit the proliferation of A549 cell line in varying degrees, and the mechanism may down-regulate the expression of Bcl-2, so as to induce apoptosis of human lung adenocarcinoma cancer cell A549.