艾灸对结肠炎相关性结肠癌大鼠细胞增殖相关蛋白PCNA、CyclinD1的影响
Effect of Moxibustion on Cell Proliferation-Related Proteins PCNA and CyclinD1 in Colitis-Associated
DOI: 10.12677/TCM.2021.102022, PDF,    国家自然科学基金支持
作者: 李昆珊, 林亚莹, 赵继梦, 李国娜, 李 琪, 黄 艳：上海市针灸经络研究所，上海；上海中医药大学附属岳阳中西医结合医院，上海；李灵杰, 陈珂旭：上海中医药大学，上海；王照钦：上海市针灸经络研究所，上海
关键词: 结肠炎相关性结肠癌；艾灸；增殖细胞核抗原；细胞周期蛋白D1；细胞增殖；Colitis-Associated Cancer； Moxibustion； Proliferating Cell Nuclear Antigen； CyclinD1； Cell Proliferation
摘要: 目的：观察艾灸“天枢、气海”穴对结肠炎相关性结肠癌(CAC)模型大鼠肿瘤的影响，从细胞增殖角度探讨艾灸对CAC的作用机制。方法：将SD大鼠随机分为正常组、模型组、隔药灸组、隔姜灸组。采用AOM/DSS方法构建CAC模型，隔药灸组和隔姜灸组选用“天枢、气海”穴，每穴灸2壮/次，共30次。观察大鼠结肠组织成瘤率、组织病理学改变和肿瘤评分；ELISA法检测血清CysC含量；免疫组化法、Western blot法检测结肠组织PCNA和CyclinD1蛋白表达水平。结果：与正常组比，模型组成瘤率和肿瘤评分均升高(P < 0.05)，光镜下结肠组织可见不同级别异型增生，达到腺癌程度；血清CysC含量、结肠组织PCNA和CyclinD1蛋白的表达均显著升高(P < 0.05)。与模型组比，隔药灸组、隔姜灸组成瘤率和肿瘤评分均降低(P < 0.05)，血清CysC含量、结肠组织PCNA和CyclinD1蛋白的表达均显著降低(P < 0.05)。结论：隔药灸和隔姜灸可延缓CAC肿瘤生长，其机制可能与降低结肠组织PCNA和CyclinD1蛋白的表达，从而抑制结肠细胞增殖相关。
Abstract: Objective: To observe the effect of moxibustion “Tianshu” and “Qihai” on the growth of colon tu-mors in colitis-associated cancer (CAC) model rats, and explore the mechanism of moxibustion in CAC from the perspective of cell proliferation. Methods: SD rats were randomly divided into normal group, model group, herb-partitioned moxibustion group and ginger-partitioned moxibustion group. The CAC model was constructed by AOM/DSS method. Herb-partitioned moxibustion group and ginger-partitioned moxibustion group used moxibustion “Tianshu” and “Qihai”, with 2 times per pointeach time, a total of 30 times. The tumor formation rate, histopathological changes and tumor score of colon tissues were observed. Serum CysC was detected by ELISA. The expression of PCNA and CyclinD1 proteins in colon tissues was detected by immunohistochemistry and Western blot. Results: Compared with normal group, the tumor formation rate and tumor score of the colon tissues in model group were both increased (P < 0.05), and different grades of dysplasia can be seen in colon tissue under light microscope, reaching the level of adenocarcinoma. The content of serum CysC, the expression of PCNA and CyclinD1 proteins in colon tissues were significantly increased (P < 0.05). Compared with model group, the tumor formation rate and tumor score of the colon tissues in herb-partitioned moxibustion group and ginger-partitioned moxibustion group were both reduced (P < 0.05). The content of serum CysC, the expression of PCNA and CyclinD1 protein in colon tissues were significantly decreased (P < 0.05). Conclusion: Herb-partitioned moxibustion and ginger-partitioned moxibustion can delay the growth of CAC tumors, and their mechanism may be related to inhibit the cell proliferation of colon tissue by reducing the expression of PCNA and CyclinD1 proteins.
文章引用：李昆珊, 林亚莹, 李灵杰, 陈珂旭, 赵继梦, 李国娜, 王照钦, 李琪, 黄艳. 艾灸对结肠炎相关性结肠癌大鼠细胞增殖相关蛋白PCNA、CyclinD1的影响[J]. 中医学, 2021, 10(2): 157-168. https://doi.org/10.12677/TCM.2021.102022

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