橡胶草幼叶与成熟叶片转录组比较分析

Comparative Transcriptome Analysis of Young and Mature Leaves of Taraxacum kok-saghyz L. Rodin

摘要: 橡胶草(Taraxacum kok-saghyz L. Rodin)是天然橡胶产业最有发展潜力的新型产胶替代作物。除产胶之外它还能够产生菊粉等大量的次生代谢产物。为了进一步阐明橡胶草叶片中次生代谢产物合成的分子机制，本研究以新疆橡胶草幼叶和成熟叶片为材料，采用华大BGISEQ-500平台进行转录组测序，分别得到165,935,798条和166,460,570条的clean reads，并注释到Nr、KOG、KEGG、InterPro和Swiss-Prot数据库中。Unigene共有58,402条被注释结果，通过差异基因表达分析、GO分类和KEGG代谢通路分析，代谢通路分属于136类，其中包括倍萜类化合物、核糖体、黄酮醇和花青素等次生代谢物质生物合成，最后进行差异基因的蛋白互作分析。该研究结果对橡胶草叶片药用活性成分生物的合成与代谢、关键酶基因的克隆以及分子标记开发等研究有一定的参考价值。

Abstract: Rubber grass (Taraxacum kok-saghyz L. Rodin) is the most promising rubber-producing alternative crop for new natural rubber. It can biosynthesize large amounts of secondary metabolites such as inulin. In order to further clarify the molecular mechanism of secondary metabolite biosynthesis in rubber grass leaves, this study used young and mature leaves of Xinjiang rubber grass as materials, and used the BGISEQ-500 platform for transcriptome sequencing to obtain 165,935,798 and 166,460,570 clean reads, respectively. Annotate to Nr, KOG, KEGG, InterPro and Swiss-Prot databases. Unigene has a total of 58,402 annotated results, and then performed differential gene expression analysis, GO classification, and KEGG metabolic pathway analysis. The metabolic pathways are classified into 136 categories, including the synthesis of sesquiterpenoids, ribosomes, flavonols, anthocyanins, etc., biosynthesis of secondary metabolites, and finally protein-protein interaction analysis of differential genes. The results of this study have certain reference value for the research on the biosynthesis and metabolism of medicinal active ingredients in rubber grass leaves, the cloning of key enzyme genes, and the development of molecular markers.