丹参酮IIA对内毒素致急性肺损伤大鼠的影响

Effects of Tanhinone IIA on Mice with Endotoxin-Caused Acute Lung Injury

摘要: 目的：通过观察研究丹参酮IIA作用于内毒素导致的急性肺损伤大鼠后相关炎症因子及氧合指标的影响，从而探讨该药对急性肺损伤的治疗效果。方法：选取成年健康大鼠40只，采用气道内滴注脂多糖(LPS)来建立急性肺损伤(ALI)的大鼠模型，随机分为4组：生理盐水对照组，LPS组，丹参酮IIA + LPS观察组，血必净 + LPS观察组，在治疗6 h，12 h后，观察各组大鼠动脉血氧分压(PaO2)、支气管肺泡灌洗液(BALF)中肿瘤坏死因子-α (TNF-α)，及细胞总数，中性粒细胞计数的改变。结果：LPS组大鼠中动脉血氧分压较对照组均明显降低，丹参酮IIA + LPS组和血必净 + LPS组也降低，与LPS组相比明显升高(P < 0.05)，丹参酮IIA + LPS组和血必净 + LPS组相比无统计学差异(P > 0.05)；LPS组大鼠中细胞总数及中性粒细胞计数升高，与对照组相比(P < 0.05)，丹参酮IIA + LPS组和血必净 + LPS组也升高，但与LPS组相比明显降低(P < 0.05)，丹参酮IIA + LPS组和血必净 + LPS组相比无统计学差异(P > 0.05)；LPS组支气管肺泡灌洗液中肿瘤坏死因子-α (TNF-α)活性较正常大鼠升高，丹参酮IIA + LPS组，血必净 + LPS组的肿瘤坏死因子-α (TNF-α)活性较LPS组降低(P < 0.05)。结论：丹参酮IIA能够下调内毒素导致的急性肺损伤大鼠的炎症因子水平，缓解急性肺损伤的炎症反应，从而对急性肺损伤起到治疗效果。

Abstract: Objective: By observing study of tanshinone IIA role in endotoxin induced acute lung injury in rats after related inflammatory factor and oxygenation index, explore the effect of the drug in the treatment of acute lung injury. Methods: Selected 40 adult health rats, established the rat model of acute lung injury (ALI) by using the lipopolysaccharide (LPS) airway instillation, divided into 4 groups randomly: normal control group, LPS group, tanshinone IIA + LPS group, Xuebijing + LPS group. Observing rat arterial blood oxygen partial pressure (PaO2), tumor necrosis factor-α (TNF-α) in bronchoalveolar lavage fluid, and the total number of cells, and neutrophils count change after treatment 6 h, 12 h. Result: The arterial blood oxygen pressure of the LPS group was significantly lower than the control group, and the level of the tanshinone IIA + LPS group and the Xuebijing + LPS group were also reduced, but significantly higher than in the LPS group (P < 0.05), no statistical difference between the tanshinone IIA + LPS group and the Xuebijing + LPS group (P > 0.05). The total number of cells and neutrophil count were increased compared with control group (P < 0.05). Tumor necrosis factor-alpha (TNF-alpha) activity in the BALF in the LPS group was higher than in normal rats, the activity of tumor necrosis in the tanshinone IIA + LPS group and the Xuebijing +LPS group were lower than that of the LPS group (P < 0.05). Conclusion: Tanshinone IIA can reduce the level of inflammatory factor in mice with acute lung injury caused by endotoxin, relieve the inflammatory response of acute lung injury, and have therapeutic effect on acute lung injury.