盐霉素对胶质母细胞瘤U87细胞抑制作用的研究

Studying of Inhibitory Effect of Salinomycin on Glioblastoma U87 Cells

摘要: 目的：观察盐霉素对人胶质母细胞瘤(U87)细胞增殖、凋亡的影响，检测盐霉素对凋亡相关基因表达影响以探究对U87细胞抑制机制。方法；分别用高浓度(SH, 4 μmol/L)、低浓度盐霉素(SL, 2 μmol/L)处理U87细胞，分为空白对照组(NC组)、SH组、SL组，48小时后，通过集落形成实验观察盐霉素对U87细胞的集落形成能力的影响。通过RT-PCR检测SENP2等33个凋亡相关下调基因RNA的表达水平。结果：集落形成实验结果显示，SH和SL组均能抑制U87的增殖，且成浓度依赖性(P < 0.05)。PCR结果得到SENP2等33个凋亡相关基因表达下调。结论；盐霉素对U87细胞增殖具有明显抑制作用，并促进细胞凋亡。

Abstract: Objective: To observe the effect of salinomycin on the proliferation and apoptosis of human glio-blastoma U87 cells, and to detect the effect of salinomycin on the expression of apoptosis-related genes to explore the mechanism of inhibition on U87 cells. Methods: U87 cells were treated with high concentration (SH, 4 μmol/L) and low concentration salinomycin (SL, 2 μmol/L), and divided into NC group, SH group, and SL group. After 48 hours, observe the effect of salinomycin on the col-ony forming ability of U87 cells through the colony formation experiment. RT-PCR was used to de-tect the RNA expression level of 33 apoptosis-related down-regulated genes such as SENP2. Results: The results of MTT and colony formation experiments showed that both SH and SL groups could in-hibit the proliferation of U87 in a concentration-dependent manner (P < 0.05). Flow cytometry re-sults showed that the apoptosis rate in SH group increased. PCR results showed that the expression of 33 apoptosis-related genes such as SENP2 was down-regulated. Conclusion: Salinomycin can sig-nificantly inhibit the proliferation of U87 cells and promote cell apoptosis.