基于网络药理学方法揭示山茱萸作用于骨质疏松的机制

Study on Mechanism of Cornus officinalis against Osteoporosis Based on Network Pharmacology

摘要: 目的：基于网络药理学与生物信息学方法筛选山茱萸有效活性成分和潜在作用靶点，构建山茱萸活性成分–作用靶点网络图及蛋白互相作用(protein-protein-interaction network, PPI network)网络图。方法：通过中药系统药理学数据库(TCMSP)检索山茱萸主要活性成分及其潜在的作用靶点，通过OMIM和Genecards数据库检索骨质疏松相关靶点基因，利用R软件绘制山茱萸靶点基因与骨质疏松靶点基因的韦恩图。利用Cytoscape3.7.2筛选核心网络，通过STRING数据库构建PPI网络，并进行GO功能、KEGG通路的富集分析。结果：本研究经检索并通过筛选后可得到山茱萸活性成分12个，与骨质疏松相关的靶点基因30个。并且得到GO功能富集中生物过程485个条目，细胞组分36个条目，分子功能58个条目。KEGG富集分析共映射出59条信号通路。结论：通过生物信息学及网络药理学揭示了山茱萸有效活性成分——多靶点基因–多通络治疗骨质疏松的分子作用机制。

Abstract: Objective: To explore the molecular network regulation mechanism of Cornus officinalis in osteoporosis, this study is based on network pharmacology and bioinformatics methods to screen effective active ingredients and potential targets of Cornus officinalis, then construct an active component-action target network diagram and protein-protein-interaction network (PPI) diagram of Cornus officinalis. Methods: The main active ingredients and potential targets of Cornus officinalis were retrieved and screened through the Chinese Medicine System Pharmacology Database and Analysis Platform (TCMSP). Target genes of osteoporosis were retrieved through the OMIM and Genecards databases. The R software was used to draw the Venn diagram which was about target genes of Cornus officinalis and osteoporosis. Perl software and Cytoscape 3.7.2 were used to construct the active ingredient-action target network diagram of Cornus officinalis. The PPI network was constructed through the STRING database, and the core genes of the PPI network were extracted using R software. And we used R language to analyse GO function and KEGG pathway. Results: After screening OB and DL in this study, 12 active ingredients of Cornus officinalis can be obtained. We found that there are 30 target genes related to osteoporosis among the active ingredient targets. In this study, there were 485 entries in the biological process, 36 entries in cell component analysis, and 58 entries in molecular function analysis. In addition, KEGG enrichment analysis has 59 signal pathways. Conclusion: Based on bioinformatics and network pharmacology, it revealed the molecular mechanism of Cornus officinalis effective active ingredient-multi-target gene-multi-channel network in the treatment of osteoporosis.