IDO在烟曲霉菌性角膜炎中的免疫调节作用

Indoleamine 2,3-Dioxygenase Adjusts Immunization in Aspergillus Fumigatus Keratitis

摘要: 吲哚胺2,3-双加氧酶(Indoleamine 2,3-dioxygenase, IDO)是一种具有免疫调节功能并参与色氨酸降解的限速酶，可以通过影响巨噬细胞作用调节炎症反应中的免疫应答，但IDO在烟曲霉菌性角膜炎中的其他免疫调节作用及机制仍不十分明了，因此，本实验在烟曲霉菌感染的小鼠模型及中性粒细胞模型上进行研究。方法：建立小鼠真菌性角膜炎动物模型，记录小鼠角膜炎症评分；采用RT-PCR检测烟曲霉菌菌丝刺激不同时间点的小鼠中性粒细胞中IDO、IDO下游因子AhR及炎症因子(TNF-α, IL-1β, IL-6, IL-10, IL-12) mRNA的表达。采用RT-PCR检测烟曲霉菌灭活菌丝刺激中性粒细胞16小时后各组炎症因子(IL-1β, TNF-α, IL-6) mRNA的表达变化。

Abstract: Indoleamine 2,3-dioxygenase (IDO) is a rate-limiting enzyme that functions as an immunomodulator and participates in the degradation of tryptophan. IDO can regulate the immune response of inflammation by affecting the action of macrophages. However, the immunomodulatory role and mechanism of IDO in Aspergillus fumigatus keratitis are still not very clear. Therefore, this study was conducted on the mouse model of Aspergillus fumigatus infection and the neutrophils model. METHODS: Animal models were developed to measure inflammatory scores and RT-PCR was used to detect the mRNA expression of IDO, downstream factors AHRs of IDO and inflammatory factors (TNF-α, IL-1β, IL-6, IL-10, IL-12) in the cornea of mice stimulated by Aspergillus niacae at different time points. RT-PCR was used to detect the mRNA expression of inflammatory factors (IL-1β, TNF-α, IL-6) in each group after 16 hours of stimulation by Aspergillus fumigatus inactivated hyphae.