大豆分枝数QTL定位及候选基因挖掘
QTL Analysis and Candidate Gene Mining of Soybean Branch Number
DOI: 10.12677/HJAS.2021.115062, PDF,   
作者: 韩 雪：黑龙江八一农垦大学，黑龙江 大庆;东北农业大学，黑龙江 哈尔滨；万晨茜, 齐照明：东北农业大学，黑龙江 哈尔滨
关键词: 大豆；分枝；QTL；候选基因；Soybean； Branches Number； QTL； Candidate Genes
摘要: 大豆分枝数与大豆产量密切相关，因此对大豆分枝数进行QTL精细定位及对其候选基因的挖掘在生产实际中具有重要意义。本研究利用SLAF测序技术，针对一套RIL群体(亲本：Charleston (♀)和东农594 (♂))构建了大豆的高密度遗传图谱，分别采用复合区间作图法(CIM)及完备区间作图法(ICIM)对4个环境的大豆分枝数表现数据进行QTL初步定位。获得10个大豆分枝数相关的QTL位点，并利用生物信息工具对区间内基因进行功能注释，筛选到与大豆分枝数相关的候选基因有20个，分别为Glyma01g37051，Glyma02g05350，Glyma02g06830，Glyma02g10540，Glyma19g07557，Glyma02g09650，Glyma02g03990，Glyma02g07940，Glyma02g14125，Glyma02g14910，Glyma02g13053，Glyma02g08680，Glyma02g14450，Glyma02g11850，Glyma02g15400，Glyma02g12700，Glyma13g14002，Glyma02g10910，Glyma02g15380，Glyma02g15390。结论：为大豆高产育种策略的制定提供有利保证，加快品种定向选育，缩短育种时间，提高育种实效，育成品种可以尽快进入产业化生产。
Abstract: Soybean branches number is closely related to soybean yield, so it is very important to fine map QTL for the number of branches and explores candidate genes in soybean production. In this study, we used SLAF sequencing technology to target a RIL population (parent: Charleston) (♀) He Dongnong 594 (♂) A high-density genetic map of soybean was constructed, and QTLs of branch number of soybean in four environments were mapped by CIM and ICIM respectively. Ten main effect QTL loci related to the number of branches in soybean were obtained, and 20 candidate genes related to the number of branches were screened by bioinformatics tools which were Glyma01g37051, Glyma02g05350, Glyma02g06830, Glyma02g10540, Glyma19g07557, Glyma02g09650, Glyma02g03990, Glyma02g07940, Glyma02g14125, Glyma02g14910, Glyma02g13053, Glyma02g08680, Glyma02g- 14450, Glyma02g11850, Glyma02g15400, Glyma02g12700, Glyma13g14002, Glyma02g10910, Glyma02g15380, Glyma02g15390. It can provide a favorable guarantee for the formulation of high-yield breeding strategy, speed up the directional breeding of soybean varieties, improve the breeding effect, and the new varieties can enter the industrial production as soon as possible.
文章引用：韩雪, 万晨茜, 齐照明. 大豆分枝数QTL定位及候选基因挖掘[J]. 农业科学, 2021, 11(5): 449-455. https://doi.org/10.12677/HJAS.2021.115062

参考文献

[1] 王志贤. 大豆产量相关性状的遗传与稳定性分析及QTL定位研究[D]: [硕士学位论文]. 北京: 中国农业科学院, 2008.
[2] 杨凤娇. 禾本科植物分蘖为茎的分枝研究提供新视角[J]. 热带亚热带植物学报, 2015, 23(2): 218-226.
[3] Qi, Z.M., Huang, L., Zhu, R.S., Xin, D.W., Liu, C.Y., Han, X., Jiang, H.W., Hong, W.G., Hu, G.H., Zheng, H.K. and Chen, Q.S. (2014) A High-Density Genetic Map for Soybean Based on Specific Length Amplified Fragment Sequencing. PLOS ONE, 9, e104871. [Google Scholar] [CrossRef] [PubMed]
[4] 宋显君. 基于SSR标记的大豆遗传图谱构建与重要农艺性状QTL定位[D]: [硕士学位论文]. 沈阳: 沈阳农业大学, 2007.
[5] 王珍. 大豆SSR遗传图谱构建及重要农艺性状QTL分析[D]: [硕士学位论文]. 南宁: 广西大学, 2004.
[6] 朱晓丽. 大豆遗传图谱构建及在两个群体重要农艺性状的QTL定位[D]: [硕士学位论文]. 哈尔滨: 东北农业大学, 2006.
[7] 杨竹丽, 李贵全. 晋大52×晋大57 RIL群体重要农艺性状的QTL定位[J]. 华北农学报, 2010, 25(2): 88-92.
[8] 程立国. 大豆遗传图谱构建和重要性状的QTL定位[D]: [硕士学位论文]. 南京: 南京农业大学, 2008.
[9] 蒋春志, 裴翠娟, 荆慧贤, 张孟臣, 王涛, 邸锐, 刘兵强, 闫龙. 大豆品质及农艺性状的QTL分析[J]. 华北农学报, 2011, 26(5): 127-130.
[10] 位艳丽. 大豆农艺和品质性状遗传模型分析与QTL定位[D]: [硕士学位论文]. 郑州: 河南农业大学, 2011.
[11] 黄中文, 赵团结, 喻德跃, 陈受宜, 盖钧镒. 大豆产量有关性状QTL的检测[J]. 中国农业科学, 2009, 42(12): 4155-4165.
[12] Isebrands, J.G. and Nelson, N.D. (1982) Crown Architecture of Short-Rotation, Intensively Cultured Populus II. Branch Morphology and Distribution of Leaves within the Crown of Populus “Tristis” as Related to Biomass Production. Canadian Journal of Forest Research, 344, 853-864. [Google Scholar] [CrossRef] 
[13] Kauppi, A., Kiviniitty, M. and Ferm, A. (1988) Growth Habits and Crown Architecture of Betula pubescens Ehrh. of Seed and Sprout Origin. Canadian Journal of Forest Research, 18, 1603-1613. [Google Scholar] [CrossRef] 
[14] Mizumachi, E., Mori, A., Osawa, N., Akiyama, R. and Tokuchi, N. (2006) Shoot Development and Extension of Quercus serrata Saplings in Response to Insect Damage and Nutrient Conditions. Annals of Botany, 98, 219-226. [Google Scholar] [CrossRef] [PubMed]
[15] 陈庆山, 张忠臣, 刘春燕, 辛大伟, 单大鹏, 邱红梅, 单彩云. 大豆主要农艺性状的QTL分析[J]. 中国农业科学, 2007, 40(1): 41-47.
[16] 周蓉, 王贤智, 陈海峰, 张晓娟, 单志慧, 吴学军, 蔡淑平, 邱德珍, 周新安, 吴江生. 大豆倒伏性及其相相关性状的QTL分析[J]. 作物学报, 2009, 35(1): 57-65.
[17] 何冉, 关荣霞, 刘章雄, 朱晓丽, 常汝镇, 邱丽娟. 用分离群体中的残余杂合系定位大豆C1连锁群的分枝数qBN-c1-1位点[J]. 中国农业科学, 2009, 42(4): 1152-1157.
[18] Sayama, T., Hwang, T.Y., Yamazaki, H., Yamaguchi, N., Komatsu, K., Takahashi, M., Suzuki, C., Miyoshi, T., Tanaka, Y., Xia, Z., Tsubokura, Y., Watanabe, S., Harada, K., Funatsuki, H. and Ishimoto, M. (2010) Mapping and Comparison of Quantitative Trait Loci for Soybean Branching Phenotype in Two Locations. Breeding Science, 60, 380-389. [Google Scholar] [CrossRef] 
[19] Li, W., Zheng, D.H., Van, K., et al. (2008) QTL Mapping for Major Agronomic Traits across Two Years in Soybean (Glycine max L. Merr.). Journal of Crop Science and Biotechnology, 11, 171-190.
[20] Nelson, R. (1996) The Inheritance of a Branching Type in Soybean. Crop Science, 36, 1150-1152. [Google Scholar] [CrossRef] 
[21] Maughan, P.J., Maroof, M.A.S. and Buss, G.R. (1996) Molecular-Marker Analysis of Seed-Weight: Genomic Locations, Gene Action, and Evidence for Ortholo-gous Evolution among Three Legume Species. Theoretical and Applied Genetics, 93, 574-579. [Google Scholar] [CrossRef] 
[22] Keim, P., Diers, B.W., Olson, T.C. and Shoemaker, R.C. (1990) RFLP Mapping in Soybean: Association between Marker Loci and Variation in Quantitative Traits. Genetics, 126, 735-742. [Google Scholar] [CrossRef] [PubMed]
[23] Orf, J.H., Chase, K., Jarvik, T., Mansur, L.M., Cregan, P.B., Adler, F.R. and Lark, K.G. (1999) Genetics of Soybean Agronomic Traits: I. Comparison of Three Related Recom-binant Inbred Populations. Crop Science, 39, 1652-1656. [Google Scholar] [CrossRef] 
[24] 王永军, 吴晓雷, 喻德跃, 章元明, 陈受宜, 盖钧镒. 重组自交系群体的检测调整方法及其在大豆NJRIKY群体的应用[J]. 作物学报, 2004, 30(5): 413-418.
[25] 谭冰, 郭勇, 邱丽娟. 大豆全基因组分枝相关基因发掘及与QTL共定位[J]. 遗传, 2013, 35(6): 793-804.
[26] 夏正俊. 大豆光周期反应与生育期基因研究进展[J]. 作物学报, 2013, 39(4): 571-579.
[27] 许振柱, 周广胜. 植物氮代谢及其环境调节研究进展[J]. 应用生态学报, 2004, 15(3): 511-516.
[28] 李泽琴, 李静晓, 张根发. 植物抗坏血酸过氧化物酶的表达调控以及对非生物胁迫的耐受作用[J]. 遗传, 2013, 35(1): 45-54.
[29] Wannes, V., Hilde, N., Ru-ben, V., et al. (2016) Overexpression of GA20-OXIDASE1 Impacts Plant Height, Biomass Allocation and Saccharification Efficiency in Maize. Plant Biotechnology Journal, 14, 997-1007.
[30] 王博慧, 孙晓霞, 牛建新. 库尔勒香梨kfpMYB及其启动子的克隆和对激素的应答反应[J]. 园艺学报, 2015, 42(8): 1448-1456.
[31] Paterson, A.H., Lander, E.S., Hewitt, J.D., et al. (1988) Resolution of Quantitative Traits into Mende-lian Factors by Using a Complete Linkage Map of Restriction Fragment Length Polymorphisms. Nature, 335, 721-726. [Google Scholar] [CrossRef] [PubMed]

为你推荐