烟草赤星病生防菌的筛选、鉴定及应用研究
Screening, Identification and Application of Biocontrol Bacteria against Tobacco Brown Spot Optimization
DOI: 10.12677/HJAS.2021.115064, PDF,  被引量    国家科技经费支持
作者: 杨德政, 桑维钧*：贵州大学烟草学院，贵州 贵阳；孙光军：中国烟草总公司贵州省公司烟叶处，贵州 贵阳
关键词: 烟草赤星病；生防菌；筛选鉴定；防治效果；Tobacco Brown Spot Disease； Biocontrol； Screening and Identification； Prevention and Control Effect
摘要: 为了从生物途径探索有效控制烟草赤星病对烟叶生产带来的危害，本文以烟草赤星病菌为指示菌，采用平板对峙法从烟草根际土壤中筛选出了烟草赤星病的一种拮抗菌Y2，通过形态特征、生理生化特征及16S rRNA、gyrA序列分析等手段，鉴定该菌株为贝莱斯芽孢杆菌(Bacillus velezensis)。之后，利用该拮抗菌株分别进行了烟草赤星病离体叶片与盆栽防效试验，结果发现其对烟草赤星病的防效分别为57.17%和59.81%。另外，研究表明该菌株对其他4种烟草病害病原菌也存在不同程度的抑制作用。研究结果为该拮抗菌株的进一步研究奠定了基础，并且为烟草赤星病的生物防治提供了参考。
Abstract: In order to explore the effective control method to tobacco brown spot disease from the biological way, an antagonistic bacteria strain Y2 was isolated from tobacco rhizosphere soil by plate confrontation method, with an Alternaria alternata strain as indicator. The inhibition rate to A. alternata was 51.5%. By means of morphological, physiological and biochemical characteristics, as well as 16S rRNA and gyrA sequence analysis, the Y2 strain was finally identified as Bacillus velezensis. The control effects of Y2 strain on tobacco brown spot In vitro leaf experiment and pot experiment were 57.17% and 59.81%, respectively. At the same time, it was found that Y2 strain had different degrees of inhibition also on four tobacco disease pathogens. The results of the experiment laid a foundation for further study on the antagonistic bacteria on tobacco disease.
文章引用：杨德政, 孙光军, 桑维钧. 烟草赤星病生防菌的筛选、鉴定及应用研究[J]. 农业科学, 2021, 11(5): 461-469. https://doi.org/10.12677/HJAS.2021.115064

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