NGS对下呼吸道感染诊断价值的探讨

A Review of the Value of Next-Generation Sequencing in the Diagnosis of Lower Respiratory Tract Infection

摘要: 下呼吸道感染作为目前导致人类死亡的第三大原因，其发病率、死亡率高，是全世界认可的常见呼吸系统疾病，危害性巨大。其病原微生物因地域、民族存在差异而不尽相同。在全球范围内，临床诊断病原体有效率低、新发传染病及少见病频出、菌群耐药等问题。因此精确的病原体诊断成为解决上述问题的关键。近年来，二代测序技术(NGS)作为一种新型检测病原体手段，在临床上被广泛应用，为我们明确病原体、拓展对微生物的认知以及临床诊治提供了一条新途径。本文就二代测序技术在下呼吸道感染中的研究现状及前景进行综述。

Abstract: As the third leading cause of human death, lower respiratory tract infection (LRTIs) is a globally recognized common respiratory disease with high morbidity and mortality. The pathogenic microorganisms are different when there are differences in regions and nationalities. In the world, there are some problems, such as low efficiency of clinical diagnosis of pathogens, frequent occurrence of new and rare diseases, drug resistance of bacteria and so on. Therefore, accurate diagnosis of pathogens is the key to solve the above problems. In recent years, the next-generation sequencing (NGS) technology, as a new method to detect pathogens, has been widely used in clinic, which provides a new way for us to identify pathogens, expand our understanding of microorganisms and provide a new way for clinical diagnosis and treatment. This article reviews the research status and prospect of next-generation sequencing technology in lower respiratory tract infection.