白细胞介素6可能通过Jak2诱导平滑肌细胞成骨样分化及钙化
Interleukin 6 May Induce Osteogenic Differentiation and Calcification of Vascular Smooth Muscle Cells through Jak2
摘要: 目的：探讨IL6诱导平滑肌细胞钙化及成骨样分化的机制。方法：体外培养平滑肌细胞，分别给予IL6、IL6 + CP690550干预细胞，设空白对照。免疫印迹法(Western blot)检测钙化相关分子TNAP、转录因子Runx2、Jak2的表达，邻甲酚酞法(O-cresolphthalein)检测不同时间的钙盐含量。结果：IL6组较空白组，钙盐含量明显高于对照组，同时TNAP、Runx2、Jak2的表达明显上调。给予Jak2抑制剂后，较空白组TNAP、Runx2表达明显下调。结论：IL6可能通过Jak2诱导平滑肌细胞成骨样分化及钙化。
Abstract: Objective: To investigate the mechanism of IL6 inducing calcification and osteogenic differentiation of vascular smooth muscle cells. Methods: Vascular smooth muscle cells were cultured in vitro, IL6 and IL6 + CP690550 were given to the intervention cells respectively, and a blank control was set. Western blotting was used to detect the expression of calcification-related molecules TNAP, transcription factor Runx2 and Jak2, and the o-cresolphthalein method was used to detect the calcium salt content at different times. Results: Compared with the blank group, the IL6 group had significantly higher calcium content than the control group, and the expressions of TNAP, Runx2 and Jak2 were significantly up-regulated. After adding Jak2 inhibitor, the expressions of TNAP and Runx2 were significantly down-regulated compared with the blank group. Conclusion: IL6 may induce osteogenic differentiation and calcification of vascular smooth muscle cells through Jak2.
文章引用：郭欣欣, 曲一丹, 赵旋. 白细胞介素6可能通过Jak2诱导平滑肌细胞成骨样分化及钙化[J]. 临床医学进展, 2021, 11(6): 2576-2581. https://doi.org/10.12677/ACM.2021.116371

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