新型微小RNA-9对乳腺癌增殖的影响及靶基因预测的生信分析

Effects of Novel MicroRNA-9 on Breast Cancer Proliferation and Bioinformatics Analysis of Target Gene Prediction

摘要: 目的：通过对乳腺癌组织测序发现novel-miR-9在癌组织中呈现低表达。深入研究novel-miR-9对乳腺癌的调控机制及其生物学功能理论机制。方法：运用cck-8以及EdU实验检测novel-miR-9对人乳腺癌细胞增殖的影响；应用生物信息学分析预测novel-miR-9靶基因以及与乳腺癌相关基因；用Venny2.1.0绘制韦恩图得到靶基因集合；对靶基因集合进行GO功能注释分析，找出与细胞增殖相关基因；分析其基因在乳腺癌表达量；并对其进行蛋白交互作用，GO功能注释分析和KEGG Pathway分析。结果：novel-miR-9在人乳腺癌细胞呈低表达(P < 0.001)；抑制乳腺癌细胞增殖能力(P < 0.001)；通过Venny图以及GO功能注释分析和蛋白交互作用分析找出与细胞增殖相关的26个基因并发现其相互作用关系较复杂；GO分析发现靶基因可能参与细胞增殖、信号接收等生物过程；KEGG Pathway分析发现其靶基因主要富集在PI3K-Akt、Ras、癌症、癌症中的microRNA等信号通路。结论：novel-miR-9调控参与多种重要的生物学过程，为后续研究提供了线索。

Abstract: Objective: to study the low expression of novel-miR-9 in breast cancer by sequencing. For the further study of novel-miR-9 regulation mechanism of breast cancer and its biological function theory mechanism. Methods: cck-8 and EdU experiments were used to detect the effect of novel-miR-9 on human breast cancer cell proliferation; application of bioinformatics analysis to predict novel-miR-9 target genes and breast cancer related genes; a set of target genes was obtained by drawing Wayne map with Venny2.1.0; GO functional annotation analysis of target gene sets, Identification of genes related to cell proliferation; to analyze the expression of its gene in breast cancer; and protein interaction, GO function annotation analysis and KEGG Pathway analysis. Results: novel-miR-9 low expression in human breast cancer cells (P < 0.001); inhibition of breast cancer cell proliferation (P < 0.001); through Venny map and GO function annotation analysis and protein interaction analysis to identify 26 genes related to cell proliferation and found that the interaction relationship is more complex; GO analysis found that target genes may be involved in cell proliferation, signal reception and other biological processes; KEGG Pathway analysis found that its target genes are most enriched in PI3K-Akt, Ras, cancer, cancer microRNA and other signaling pathways. Conclusions: novel-miR-9 regulation is involved in many important biological processes, provided clues for the follow-up study.